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Title: Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells

Abstract

Wnt-10b was originally isolated from lymphoid tissue and is known to be involved in a wide range of biological actions, while recently it was found to be expressed early in the development of hair follicles. However, few studies have been conducted concerning the role of Wnt-10b with the differentiation of skin epithelial cells. To evaluate its role in epithelial differentiation, we purified Wnt-10b from the supernatant of a concanavalin A-stimulated lymphocyte culture using an affinity column and investigated its effects on the differentiation of adult mouse-derived primary skin epithelial cells (MPSEC). MPSEC cultured with Wnt-10b showed morphological changes from cuboidal to spindle-shaped with inhibited proliferation, and also obtained characteristics of the hair shaft and inner root sheath of the hair follicle, represented by red-colored Ayoub Shklar staining, and reactions to AE-13 and AE-15 as seen with immunocytology. Further, RT-PCR analysis demonstrated the expression of mRNA for keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5, in Wnt-10b-treated MPSEC. In addition, involvement of the canonical Wnt signal pathway was demonstrated by a TCF reporter (pTOPFLASH) assay. These results suggest that Wnt-10b promotes the differentiation of MPSEC and may playmore » an important role in hair follicle development by promoting differentiation of epithelial cells.« less

Authors:
 [1];  [2];  [2];  [2]
  1. Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara (Japan). E-mail: oujix@naramed-u.ac.jp
  2. Program in Tissue Engineering and Department of Parasitology, Nara Medical University, Kashihara, Nara (Japan)
Publication Date:
OSTI Identifier:
20798904
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.02.028; PII: S0006-291X(06)00318-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; CONCANAVALIN A; HAIR; HAIR FOLLICLES; KERATIN; LYMPHOCYTES; MICE; MORPHOLOGICAL CHANGES; POLYMERASE CHAIN REACTION

Citation Formats

Ouji, Yukiteru, Yoshikawa, Masahide, Shiroi, Akira, and Ishizaka, Shigeaki. Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.02.028.
Ouji, Yukiteru, Yoshikawa, Masahide, Shiroi, Akira, & Ishizaka, Shigeaki. Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells. United States. doi:10.1016/j.bbrc.2006.02.028.
Ouji, Yukiteru, Yoshikawa, Masahide, Shiroi, Akira, and Ishizaka, Shigeaki. Fri . "Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells". United States. doi:10.1016/j.bbrc.2006.02.028.
@article{osti_20798904,
title = {Wnt-10b secreted from lymphocytes promotes differentiation of skin epithelial cells},
author = {Ouji, Yukiteru and Yoshikawa, Masahide and Shiroi, Akira and Ishizaka, Shigeaki},
abstractNote = {Wnt-10b was originally isolated from lymphoid tissue and is known to be involved in a wide range of biological actions, while recently it was found to be expressed early in the development of hair follicles. However, few studies have been conducted concerning the role of Wnt-10b with the differentiation of skin epithelial cells. To evaluate its role in epithelial differentiation, we purified Wnt-10b from the supernatant of a concanavalin A-stimulated lymphocyte culture using an affinity column and investigated its effects on the differentiation of adult mouse-derived primary skin epithelial cells (MPSEC). MPSEC cultured with Wnt-10b showed morphological changes from cuboidal to spindle-shaped with inhibited proliferation, and also obtained characteristics of the hair shaft and inner root sheath of the hair follicle, represented by red-colored Ayoub Shklar staining, and reactions to AE-13 and AE-15 as seen with immunocytology. Further, RT-PCR analysis demonstrated the expression of mRNA for keratin 1, keratin 2, loricrin, mHa5, and mHb5, in association with a decreased expression of the basal cell marker keratin 5, in Wnt-10b-treated MPSEC. In addition, involvement of the canonical Wnt signal pathway was demonstrated by a TCF reporter (pTOPFLASH) assay. These results suggest that Wnt-10b promotes the differentiation of MPSEC and may play an important role in hair follicle development by promoting differentiation of epithelial cells.},
doi = {10.1016/j.bbrc.2006.02.028},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 342,
place = {United States},
year = {Fri Apr 21 00:00:00 EDT 2006},
month = {Fri Apr 21 00:00:00 EDT 2006}
}