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Title: Cloning and partial characterization of Entamoeba histolytica PTPases

Abstract

Reversible protein tyrosine phosphorylation is an essential signal transduction mechanism that regulates cell growth, differentiation, mobility, metabolism, and survival. Two genes coding for protein tyrosine phophatases, designed EhPTPA and EhPTPB, were cloned from Entamoeba histolytica. EhPTPA and EhPTPB proteins showed amino acid sequence identity of 37%, both EhPTPases showed similarity with Dictyostelium discoideum and vertebrate trasmembranal PTPases. mRNA levels of EhPTPA gene are up-regulated in trophozoites recovered after 96 h of liver abscess development in the hamster model. EhPTPA protein expressed as a glutathione S-transferase fusion protein (GST::EhPTPA) showed enzymatic activity with p-nitrophenylphosphate as a substrate and was inhibited by PTPase inhibitors vanadate and molybdate. GST::EhPTPA protein selectively dephosphorylates a 130 kDa phosphotyrosine-containing protein in trophozoite cell lysates. EhPTPA gene codifies for a 43 kDa native protein. Up-regulation of EhPTPA expression suggests that EhPTPA may play an important role in the adaptive response of trophozoites during amoebic liver abscess development.

Authors:
 [1];  [1];  [1];  [2]
  1. Departamento de Patologia Experimental, Centro de Investigacion y de Estudios Avanzados del I.P.N., Mexico D.F, 07360 (Mexico)
  2. Departamento de Patologia Experimental, Centro de Investigacion y de Estudios Avanzados del I.P.N., Mexico D.F, 07360 (Mexico). E-mail: rosales@cinvestav.mx
Publication Date:
OSTI Identifier:
20798902
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2006.02.055; PII: S0006-291X(06)00350-0; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ABSCESSES; AMINO ACID SEQUENCE; AMOEBA; CLONING; GENE REGULATION; GENES; GLUTATHIONE; HAMSTERS; LIVER; METABOLISM; MOLYBDATES; PHOSPHORYLATION; TYROSINE

Citation Formats

Herrera-Rodriguez, Sara Elisa, Baylon-Pacheco, Lidia, Talamas-Rohana, Patricia, and Rosales-Encina, Jose Luis. Cloning and partial characterization of Entamoeba histolytica PTPases. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.02.055.
Herrera-Rodriguez, Sara Elisa, Baylon-Pacheco, Lidia, Talamas-Rohana, Patricia, & Rosales-Encina, Jose Luis. Cloning and partial characterization of Entamoeba histolytica PTPases. United States. doi:10.1016/j.bbrc.2006.02.055.
Herrera-Rodriguez, Sara Elisa, Baylon-Pacheco, Lidia, Talamas-Rohana, Patricia, and Rosales-Encina, Jose Luis. Fri . "Cloning and partial characterization of Entamoeba histolytica PTPases". United States. doi:10.1016/j.bbrc.2006.02.055.
@article{osti_20798902,
title = {Cloning and partial characterization of Entamoeba histolytica PTPases},
author = {Herrera-Rodriguez, Sara Elisa and Baylon-Pacheco, Lidia and Talamas-Rohana, Patricia and Rosales-Encina, Jose Luis},
abstractNote = {Reversible protein tyrosine phosphorylation is an essential signal transduction mechanism that regulates cell growth, differentiation, mobility, metabolism, and survival. Two genes coding for protein tyrosine phophatases, designed EhPTPA and EhPTPB, were cloned from Entamoeba histolytica. EhPTPA and EhPTPB proteins showed amino acid sequence identity of 37%, both EhPTPases showed similarity with Dictyostelium discoideum and vertebrate trasmembranal PTPases. mRNA levels of EhPTPA gene are up-regulated in trophozoites recovered after 96 h of liver abscess development in the hamster model. EhPTPA protein expressed as a glutathione S-transferase fusion protein (GST::EhPTPA) showed enzymatic activity with p-nitrophenylphosphate as a substrate and was inhibited by PTPase inhibitors vanadate and molybdate. GST::EhPTPA protein selectively dephosphorylates a 130 kDa phosphotyrosine-containing protein in trophozoite cell lysates. EhPTPA gene codifies for a 43 kDa native protein. Up-regulation of EhPTPA expression suggests that EhPTPA may play an important role in the adaptive response of trophozoites during amoebic liver abscess development.},
doi = {10.1016/j.bbrc.2006.02.055},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 342,
place = {United States},
year = {Fri Apr 21 00:00:00 EDT 2006},
month = {Fri Apr 21 00:00:00 EDT 2006}
}