Periostin is an extracellular matrix protein required for eruption of incisors in mice

doi:10.1016/j.bbrc.2006.02.016

Title: Periostin is an extracellular matrix protein required for eruption of incisors in mice

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Abstract

A characteristic tooth of rodents, the incisor continuously grows throughout life by the constant formation of dentin and enamel. Continuous eruption of the incisor is accompanied with formation of shear zone, in which the periodontal ligament is remodeled. Although the shear zone plays a role in the remodeling, its molecular biological aspect is barely understood. Here, we show that periostin is essential for formation of the shear zone. Periostin {sup -/-} mice showed an eruption disturbance of incisors. Histological observation revealed that deletion of periostin led to disappearance of the shear zone. Electron microscopy revealed that the disappearance of the shear zone resulted from a failure in digestion of collagen fibers in the periostin {sup -/-} mice. Furthermore, immunohistochemical analysis using anti-periostin antibodies demonstrated the restricted localization of periostin protein in the shear zone. Periostin is an extracellular matrix protein, and immunoelectron microscopy showed a close association of periostin with collagen fibrils in vivo. These results suggest that periostin functions in the remodeling of collagen matrix in the shear zone.

Authors:

Kii, Isao ^[1]; Amizuka, Norio ^[2]; Minqi, Li ^[2]; Kitajima, Satoshi ^[3]; Saga, Yumiko ^[4]; Kudo, Akira ^[5]

Department of Biological Information, Tokyo Institute of Technology, Yokohama 226-8501 (Japan)
Center for Transdisciplinary Research, Niigata University, Niigata 951-8514 (Japan)
Division of Cellular and Molecular Toxicology, National Institute of Health Science, Tokyo 158-8501 (Japan)
Division of Mammalian Development, National Institute of Genetics, Mishima 411-8540 (Japan)
Department of Biological Information, Tokyo Institute of Technology, Yokohama 226-8501 (Japan). E-mail: akudo@bio.titech.ac.jp

Publication Date:: Fri Apr 14 00:00:00 EDT 2006

OSTI Identifier:: 20798890

Resource Type:: Journal Article

Resource Relation:: Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.02.016; PII: S0006-291X(06)00278-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; ANTIBODIES; COLLAGEN; DENTIN; DIGESTION; ELECTRON MICROSCOPY; ENAMELS; ERUPTION; FIBERS; IN VIVO; LIGAMENTS; MICE; SHEAR; TEETH

Citation Formats


                    Kii, Isao, Amizuka, Norio, Minqi, Li, Kitajima, Satoshi, Saga, Yumiko, and Kudo, Akira. Periostin is an extracellular matrix protein required for eruption of incisors in mice.  United States: N. p., 2006. 
        Web.  doi:10.1016/j.bbrc.2006.02.016.

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                    Kii, Isao, Amizuka, Norio, Minqi, Li, Kitajima, Satoshi, Saga, Yumiko, & Kudo, Akira. Periostin is an extracellular matrix protein required for eruption of incisors in mice.  United States.  doi:10.1016/j.bbrc.2006.02.016.

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                    Kii, Isao, Amizuka, Norio, Minqi, Li, Kitajima, Satoshi, Saga, Yumiko, and Kudo, Akira. Fri .  
        "Periostin is an extracellular matrix protein required for eruption of incisors in mice".  United States.  
        doi:10.1016/j.bbrc.2006.02.016.

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@article{osti_20798890,

  title        = {Periostin is an extracellular matrix protein required for eruption of incisors in mice},

  author       = {Kii, Isao and Amizuka, Norio and Minqi, Li and Kitajima, Satoshi and Saga, Yumiko and Kudo, Akira},

  abstractNote = {A characteristic tooth of rodents, the incisor continuously grows throughout life by the constant formation of dentin and enamel. Continuous eruption of the incisor is accompanied with formation of shear zone, in which the periodontal ligament is remodeled. Although the shear zone plays a role in the remodeling, its molecular biological aspect is barely understood. Here, we show that periostin is essential for formation of the shear zone. Periostin {sup -/-} mice showed an eruption disturbance of incisors. Histological observation revealed that deletion of periostin led to disappearance of the shear zone. Electron microscopy revealed that the disappearance of the shear zone resulted from a failure in digestion of collagen fibers in the periostin {sup -/-} mice. Furthermore, immunohistochemical analysis using anti-periostin antibodies demonstrated the restricted localization of periostin protein in the shear zone. Periostin is an extracellular matrix protein, and immunoelectron microscopy showed a close association of periostin with collagen fibrils in vivo. These results suggest that periostin functions in the remodeling of collagen matrix in the shear zone.},

  doi          = {10.1016/j.bbrc.2006.02.016},

  journal      = {Biochemical and Biophysical Research Communications},

  number       = 3,

  volume       = 342,

  place        = {United States},

  year         = {Fri Apr 14 00:00:00 EDT 2006},

  month        = {Fri Apr 14 00:00:00 EDT 2006}

}

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DOI: 10.1016/j.bbrc.2006.02.016

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