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Title: AMP-activated protein kinase is a positive regulator of poly(ADP-ribose) polymerase

Abstract

AMPK acts as a cellular fuel gauge and responds to decreased cellular energy status by inhibiting ATP-consuming pathways and increasing ATP-synthesis. The aim of this study was to examine the role of AMPK in modulating poly(ADP-ribose) polymerase (PARP), a nuclear enzyme involved in maintaining chromatin structure and DNA repair. HT-29 cells infected with constitutively active AMPK demonstrated increased PARP automodification and an increase in bioNAD incorporation. AMPK and PARP co-immunoprecipitated under basal conditions and in response to H{sub 2}O{sub 2}, suggesting a physical interaction under both resting and stress-induced conditions. Incubation of PARP with purified AMPK resulted in the phosphorylation of PARP; and the inclusion of AMP as an AMPK activator potentiated PARP phosphorylation. Using immobilized PARP, the incorporation of bioNAD by PARP was dramatically increased following the addition of AMPK. These data suggest a novel role for AMPK in regulating PARP activity through a direct interaction involving phosphorylation.

Authors:
 [1];  [1];  [2]
  1. Division of Gastroenterology, University of Alberta, Edmonton, Alberta (Canada)
  2. Division of Gastroenterology, University of Alberta, Edmonton, Alberta (Canada). E-mail: Karen.madsen@ualberta.ca
Publication Date:
OSTI Identifier:
20798877
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.01.145; PII: S0006-291X(06)00242-7; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ADP; AMP; ATP; BIOLOGICAL STRESS; BIOSYNTHESIS; CHROMATIN; DNA; DNA REPAIR; ENZYMES; FUEL GAGES; HYDROGEN PEROXIDE; INTESTINES; PHOSPHORYLATION; RIBOSE

Citation Formats

Walker, John W., Jijon, Humberto B., and Madsen, Karen L.. AMP-activated protein kinase is a positive regulator of poly(ADP-ribose) polymerase. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.01.145.
Walker, John W., Jijon, Humberto B., & Madsen, Karen L.. AMP-activated protein kinase is a positive regulator of poly(ADP-ribose) polymerase. United States. doi:10.1016/j.bbrc.2006.01.145.
Walker, John W., Jijon, Humberto B., and Madsen, Karen L.. Fri . "AMP-activated protein kinase is a positive regulator of poly(ADP-ribose) polymerase". United States. doi:10.1016/j.bbrc.2006.01.145.
@article{osti_20798877,
title = {AMP-activated protein kinase is a positive regulator of poly(ADP-ribose) polymerase},
author = {Walker, John W. and Jijon, Humberto B. and Madsen, Karen L.},
abstractNote = {AMPK acts as a cellular fuel gauge and responds to decreased cellular energy status by inhibiting ATP-consuming pathways and increasing ATP-synthesis. The aim of this study was to examine the role of AMPK in modulating poly(ADP-ribose) polymerase (PARP), a nuclear enzyme involved in maintaining chromatin structure and DNA repair. HT-29 cells infected with constitutively active AMPK demonstrated increased PARP automodification and an increase in bioNAD incorporation. AMPK and PARP co-immunoprecipitated under basal conditions and in response to H{sub 2}O{sub 2}, suggesting a physical interaction under both resting and stress-induced conditions. Incubation of PARP with purified AMPK resulted in the phosphorylation of PARP; and the inclusion of AMP as an AMPK activator potentiated PARP phosphorylation. Using immobilized PARP, the incorporation of bioNAD by PARP was dramatically increased following the addition of AMPK. These data suggest a novel role for AMPK in regulating PARP activity through a direct interaction involving phosphorylation.},
doi = {10.1016/j.bbrc.2006.01.145},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 342,
place = {United States},
year = {Fri Mar 31 00:00:00 EST 2006},
month = {Fri Mar 31 00:00:00 EST 2006}
}