skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Overexpression of ABIN-2, a negative regulator of NF-{kappa}B, delays liver regeneration in the ABIN-2 transgenic mice

Abstract

Activation of NF-{kappa}B is one of the earliest responses at the start of liver regeneration, and is required for hepatocyte cell cycle progression. The A20-binding inhibitor of NF-{kappa}B activation-2, ABIN-2, is an inhibitor of NF-{kappa}B. However, its effects on hepatocyte cell cycle progression are not known and its involvement in liver regeneration has not been explored. In this study, the temporal expression pattern of the mouse ABIN-2 was studied during liver regeneration induced by partial hepatectomy. We demonstrate that ABIN-2 is rapidly and transiently induced, and expression peaked at around 8 h post-hepatectomy. To test that the inducible expression of ABIN-2 serves to regulate NF-{kappa}B during liver regeneration, transgenic mice overexpressing human ABIN-2 protein in the liver were generated. Our transgenic data demonstrated that overexpression of ABIN-2 inhibited NF-{kappa}B nuclear translocation, which peaked at around 2-4 h post-hepatectomy, and this led to an impairment of the G1/S transition as well as a delay in hepatocyte cell cycle progression of the regenerating liver. In addition, overexpression of ABIN-2 specifically inhibited endogenous ABIN-2 mRNA induction, suggesting a negative feedback mechanism for ABIN-2 expression. In conclusion, ABIN-2 may function as a negative regulator that downregulates NF-{kappa}B activation during liver regeneration.

Authors:
 [1];  [2];  [3];  [3];  [4];  [5]
  1. Faculty of Life Sciences and Institute of Genetics, National Yang-Ming University, Taipei, Taiwan (China)
  2. Department of Life Science, Chang Gung University, Taoyuan, Taiwan (China)
  3. (China)
  4. Laboratory Animal Center, Tzu-Chi University, Hualien, Taiwan (China)
  5. Faculty of Life Sciences and Institute of Genetics, National Yang-Ming University, Taipei, Taiwan (China) and Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan (China) and Division of Molecular and Genomic Medicine, National Health Research Institute, Zhunan, Taiwan (China). E-mail: tftsai@ym.edu.tw
Publication Date:
OSTI Identifier:
20798875
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.01.114; PII: S0006-291X(06)00061-1; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; BIOLOGICAL REGENERATION; CELL CYCLE; HEPATECTOMY; LIVER; PROTEINS; TRANSGENIC MICE; TRANSLOCATION

Citation Formats

Li, C.-C., Chou, C.-K., Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan, Division of Molecular and Genomic Medicine, National Health Research Institute, Zhunan, Taiwan, Wang, M.-H., and Tsai, T.-F.. Overexpression of ABIN-2, a negative regulator of NF-{kappa}B, delays liver regeneration in the ABIN-2 transgenic mice. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.01.114.
Li, C.-C., Chou, C.-K., Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan, Division of Molecular and Genomic Medicine, National Health Research Institute, Zhunan, Taiwan, Wang, M.-H., & Tsai, T.-F.. Overexpression of ABIN-2, a negative regulator of NF-{kappa}B, delays liver regeneration in the ABIN-2 transgenic mice. United States. doi:10.1016/j.bbrc.2006.01.114.
Li, C.-C., Chou, C.-K., Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan, Division of Molecular and Genomic Medicine, National Health Research Institute, Zhunan, Taiwan, Wang, M.-H., and Tsai, T.-F.. Fri . "Overexpression of ABIN-2, a negative regulator of NF-{kappa}B, delays liver regeneration in the ABIN-2 transgenic mice". United States. doi:10.1016/j.bbrc.2006.01.114.
@article{osti_20798875,
title = {Overexpression of ABIN-2, a negative regulator of NF-{kappa}B, delays liver regeneration in the ABIN-2 transgenic mice},
author = {Li, C.-C. and Chou, C.-K. and Department of Medical Research and Education, Taipei Veterans General Hospital, Taiwan and Division of Molecular and Genomic Medicine, National Health Research Institute, Zhunan, Taiwan and Wang, M.-H. and Tsai, T.-F.},
abstractNote = {Activation of NF-{kappa}B is one of the earliest responses at the start of liver regeneration, and is required for hepatocyte cell cycle progression. The A20-binding inhibitor of NF-{kappa}B activation-2, ABIN-2, is an inhibitor of NF-{kappa}B. However, its effects on hepatocyte cell cycle progression are not known and its involvement in liver regeneration has not been explored. In this study, the temporal expression pattern of the mouse ABIN-2 was studied during liver regeneration induced by partial hepatectomy. We demonstrate that ABIN-2 is rapidly and transiently induced, and expression peaked at around 8 h post-hepatectomy. To test that the inducible expression of ABIN-2 serves to regulate NF-{kappa}B during liver regeneration, transgenic mice overexpressing human ABIN-2 protein in the liver were generated. Our transgenic data demonstrated that overexpression of ABIN-2 inhibited NF-{kappa}B nuclear translocation, which peaked at around 2-4 h post-hepatectomy, and this led to an impairment of the G1/S transition as well as a delay in hepatocyte cell cycle progression of the regenerating liver. In addition, overexpression of ABIN-2 specifically inhibited endogenous ABIN-2 mRNA induction, suggesting a negative feedback mechanism for ABIN-2 expression. In conclusion, ABIN-2 may function as a negative regulator that downregulates NF-{kappa}B activation during liver regeneration.},
doi = {10.1016/j.bbrc.2006.01.114},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 342,
place = {United States},
year = {Fri Mar 31 00:00:00 EST 2006},
month = {Fri Mar 31 00:00:00 EST 2006}
}