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Title: Thymidine secretion by hybridoma and myeloma cells

Abstract

Secretion of thymidine appeared to be a common property of hybridoma and myeloma cells, but not of other cell types, which were tested. Of three hybridoma cell lines tested, all secreted thymidine in amounts resulting in the accumulation of thymidine to concentrations of 10-20 {mu}M in the culture medium. Also three of five myeloma cell lines that were analyzed secrete thymidine, but none of the other cell types that were studied. Thymidine was purified to homogeneity (4 mg purified from 3 l of culture medium) and identified as such by nuclear magnetic resonance spectroscopy. The cells that secreted thymidine showed high resistance to the growth inhibitory effect of thymidine.

Authors:
 [1];  [2];  [2];  [3]
  1. Department of Molecular Biosciences, University of Oslo, Oslo (Norway). E-mail: bjorn.spilsberg@biokjemi.uio.no
  2. Department of Chemistry, University of Oslo, Oslo (Norway)
  3. Department of Molecular Biosciences, University of Oslo, Oslo (Norway)
Publication Date:
OSTI Identifier:
20798872
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 342; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.01.120; PII: S0006-291X(06)00215-4; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL GROWTH; CULTURE MEDIA; NUCLEAR MAGNETIC RESONANCE; SECRETION; SPECTROSCOPY; THYMIDINE

Citation Formats

Spilsberg, Bjorn, Rise, Frode, Petersen, Dirk, and Nissen-Meyer, Jon. Thymidine secretion by hybridoma and myeloma cells. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.01.120.
Spilsberg, Bjorn, Rise, Frode, Petersen, Dirk, & Nissen-Meyer, Jon. Thymidine secretion by hybridoma and myeloma cells. United States. doi:10.1016/j.bbrc.2006.01.120.
Spilsberg, Bjorn, Rise, Frode, Petersen, Dirk, and Nissen-Meyer, Jon. Fri . "Thymidine secretion by hybridoma and myeloma cells". United States. doi:10.1016/j.bbrc.2006.01.120.
@article{osti_20798872,
title = {Thymidine secretion by hybridoma and myeloma cells},
author = {Spilsberg, Bjorn and Rise, Frode and Petersen, Dirk and Nissen-Meyer, Jon},
abstractNote = {Secretion of thymidine appeared to be a common property of hybridoma and myeloma cells, but not of other cell types, which were tested. Of three hybridoma cell lines tested, all secreted thymidine in amounts resulting in the accumulation of thymidine to concentrations of 10-20 {mu}M in the culture medium. Also three of five myeloma cell lines that were analyzed secrete thymidine, but none of the other cell types that were studied. Thymidine was purified to homogeneity (4 mg purified from 3 l of culture medium) and identified as such by nuclear magnetic resonance spectroscopy. The cells that secreted thymidine showed high resistance to the growth inhibitory effect of thymidine.},
doi = {10.1016/j.bbrc.2006.01.120},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 342,
place = {United States},
year = {Fri Mar 31 00:00:00 EST 2006},
month = {Fri Mar 31 00:00:00 EST 2006}
}