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Title: Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury

Abstract

Lectin-like oxidized LDL receptor-1 (LOX-1) is an oxidized LDL receptor, and its role in restenosis after angioplasty remains unknown. We used a balloon-injury model of rabbit aorta, and reverse transcription-polymerase chain reaction revealed that LOX-1 mRNA expression was modest in the non-injured aorta, reached a peak level 2 days after injury, and remained elevated until 24 weeks after injury. Immunohistochemistry and in situ hybridization showed that LOX-1 was not detected in the media of non-injured aorta but expressed in both medial and neointimal smooth muscle cells (SMC) at 2 and 24 weeks after injury. Low concentrations of ox-LDL (10 {mu}g/mL) stimulated the cultured SMC proliferation, which was inhibited by antisense oligonucleotides of LOX-1 mRNA. Double immunofluorescense staining showed the colocalization of LOX-1 and proliferating cell nuclear antigen in human restenotic lesion. These results suggest that LOX-1 mediates ox-LDL-induced SMC proliferation and plays a role in neointimal formation after vascular injury.

Authors:
 [1];  [2];  [3];  [3];  [4];  [1];  [1];  [1];  [1];  [3];  [1]
  1. Department of Cardiovascular, Respiratory and Metabolic Medicine, Graduate School of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan)
  2. Department of Cardiovascular, Respiratory and Metabolic Medicine, Graduate School of Medicine, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima 890-8520 (Japan). E-mail: miyatam@m3.kufm.kagoshima-u.ac.jp
  3. Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507 (Japan)
  4. Department of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555 (Japan)
Publication Date:
OSTI Identifier:
20798837
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 341; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.12.211; PII: S0006-291X(05)02908-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; AORTA; CELL PROLIFERATION; IN-SITU HYBRIDIZATION; INJURIES; MUSCLES; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RABBITS; RECEPTORS; TRANSCRIPTION

Citation Formats

Eto, Hideyuki, Miyata, Masaaki, Kume, Noriaki, Minami, Manabu, Itabe, Hiroyuki, Orihara, Koji, Hamasaki, Shuichi, Biro, Sadatoshi, Otsuji, Yutaka, Kita, Toru, and Tei, Chuwa. Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.12.211.
Eto, Hideyuki, Miyata, Masaaki, Kume, Noriaki, Minami, Manabu, Itabe, Hiroyuki, Orihara, Koji, Hamasaki, Shuichi, Biro, Sadatoshi, Otsuji, Yutaka, Kita, Toru, & Tei, Chuwa. Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury. United States. doi:10.1016/j.bbrc.2005.12.211.
Eto, Hideyuki, Miyata, Masaaki, Kume, Noriaki, Minami, Manabu, Itabe, Hiroyuki, Orihara, Koji, Hamasaki, Shuichi, Biro, Sadatoshi, Otsuji, Yutaka, Kita, Toru, and Tei, Chuwa. Fri . "Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury". United States. doi:10.1016/j.bbrc.2005.12.211.
@article{osti_20798837,
title = {Expression of lectin-like oxidized LDL receptor-1 in smooth muscle cells after vascular injury},
author = {Eto, Hideyuki and Miyata, Masaaki and Kume, Noriaki and Minami, Manabu and Itabe, Hiroyuki and Orihara, Koji and Hamasaki, Shuichi and Biro, Sadatoshi and Otsuji, Yutaka and Kita, Toru and Tei, Chuwa},
abstractNote = {Lectin-like oxidized LDL receptor-1 (LOX-1) is an oxidized LDL receptor, and its role in restenosis after angioplasty remains unknown. We used a balloon-injury model of rabbit aorta, and reverse transcription-polymerase chain reaction revealed that LOX-1 mRNA expression was modest in the non-injured aorta, reached a peak level 2 days after injury, and remained elevated until 24 weeks after injury. Immunohistochemistry and in situ hybridization showed that LOX-1 was not detected in the media of non-injured aorta but expressed in both medial and neointimal smooth muscle cells (SMC) at 2 and 24 weeks after injury. Low concentrations of ox-LDL (10 {mu}g/mL) stimulated the cultured SMC proliferation, which was inhibited by antisense oligonucleotides of LOX-1 mRNA. Double immunofluorescense staining showed the colocalization of LOX-1 and proliferating cell nuclear antigen in human restenotic lesion. These results suggest that LOX-1 mediates ox-LDL-induced SMC proliferation and plays a role in neointimal formation after vascular injury.},
doi = {10.1016/j.bbrc.2005.12.211},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 341,
place = {United States},
year = {Fri Mar 10 00:00:00 EST 2006},
month = {Fri Mar 10 00:00:00 EST 2006}
}