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Title: Identification of a nuclear matrix attachment region like sequence in the last intron of PI3K{gamma}

Abstract

MARs are not only the structure bases of chromatin higher order structure but also have much biological significance. In this study, the whole sequence of about 100 kb in length from BAC clone of GS1-223D4 (GI: 5931478), in which human PI3K{gamma} gene is localized, was analyzed by two online-based computer programs, MARFinder and SMARTest. A strong potential MAR was predicted in the last and largest intron of PI3K{gamma}. The predicted 2 kb MAR, we refer to PIMAR, was further analyzed through biochemical methods in vitro and in vivo. The results showed that the PIMAR could be associated with nuclear matrices from HeLa cells both in vitro and in vivo. Further reporter gene analysis showed that in the transient transfection the expression of reporter gene linked with reversed PIMAR was repressed slightly, while in stably integrated state, the luciferase reporter both linked with reversed and orientated PIMAR was enhanced greatly in NIH-3T3 and K-562. These results suggest that the PIMAR maybe has the capacity of shielding integrated heterogeneous gene from chromatin position effect. Through combination of computer program analysis with confirmation by biochemical methods, we identified, for First time, a 2 kb matrix attachment region like sequence in the last intronmore » of human PI3K{gamma}.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Department of Biochemistry and Molecular Biology, Shanghai Second Medical University, Shanghai 2000 25 (China)
  2. Department of Biochemistry and Molecular Biology, Shanghai Second Medical University, Shanghai 2000 25 (China). E-mail: sundai0@163.com
Publication Date:
OSTI Identifier:
20798836
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 341; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.12.212; PII: S0006-291X(06)00039-8; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHROMATIN; COMPUTER CODES; GENE REGULATION; GENES; HELA CELLS; IN VITRO; IN VIVO; LUCIFERASE; NUCLEAR MATRIX; TRANSIENTS

Citation Formats

Dai Bingbing, Ying Lei, Cai Rong, Li Ying, Zhang Xingqian, Lu Jian, and Qian Guanxiang. Identification of a nuclear matrix attachment region like sequence in the last intron of PI3K{gamma}. United States: N. p., 2006. Web.
Dai Bingbing, Ying Lei, Cai Rong, Li Ying, Zhang Xingqian, Lu Jian, & Qian Guanxiang. Identification of a nuclear matrix attachment region like sequence in the last intron of PI3K{gamma}. United States.
Dai Bingbing, Ying Lei, Cai Rong, Li Ying, Zhang Xingqian, Lu Jian, and Qian Guanxiang. Fri . "Identification of a nuclear matrix attachment region like sequence in the last intron of PI3K{gamma}". United States. doi:.
@article{osti_20798836,
title = {Identification of a nuclear matrix attachment region like sequence in the last intron of PI3K{gamma}},
author = {Dai Bingbing and Ying Lei and Cai Rong and Li Ying and Zhang Xingqian and Lu Jian and Qian Guanxiang},
abstractNote = {MARs are not only the structure bases of chromatin higher order structure but also have much biological significance. In this study, the whole sequence of about 100 kb in length from BAC clone of GS1-223D4 (GI: 5931478), in which human PI3K{gamma} gene is localized, was analyzed by two online-based computer programs, MARFinder and SMARTest. A strong potential MAR was predicted in the last and largest intron of PI3K{gamma}. The predicted 2 kb MAR, we refer to PIMAR, was further analyzed through biochemical methods in vitro and in vivo. The results showed that the PIMAR could be associated with nuclear matrices from HeLa cells both in vitro and in vivo. Further reporter gene analysis showed that in the transient transfection the expression of reporter gene linked with reversed PIMAR was repressed slightly, while in stably integrated state, the luciferase reporter both linked with reversed and orientated PIMAR was enhanced greatly in NIH-3T3 and K-562. These results suggest that the PIMAR maybe has the capacity of shielding integrated heterogeneous gene from chromatin position effect. Through combination of computer program analysis with confirmation by biochemical methods, we identified, for First time, a 2 kb matrix attachment region like sequence in the last intron of human PI3K{gamma}.},
doi = {},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 341,
place = {United States},
year = {Fri Mar 10 00:00:00 EST 2006},
month = {Fri Mar 10 00:00:00 EST 2006}
}