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Title: In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells

Abstract

Cardiomyocyte loss in the ischemically injured human heart often leads to irreversible defects in cardiac function. Recently, cellular cardiomyoplasty with mesenchymal stem cells, which are multipotent cells with the ability to differentiate into specialized cells under appropriate stimuli, has emerged as a new approach for repairing damaged myocardium. In the present study, the potential of human umbilical cord-derived mesenchymal stem cells to differentiate into cells with characteristics of cardiomyocyte was investigated. Mesenchymal stem cells were isolated from endothelial/subendothelial layers of the human umbilical cords using a method similar to that of human umbilical vein endothelial cell isolation. Isolated cells were characterized by transdifferentiation ability to adipocytes and osteoblasts, and also with flow cytometry analysis. After treatment with 5-azacytidine, the human umbilical cord-derived mesenchymal stem cells were morphologically transformed into cardiomyocyte-like cells and expressed cardiac differentiation markers. During the differentiation, cells were monitored by a phase contrast microscope and their morphological changes were demonstrated. Immunostaining of the differentiated cells for sarcomeric myosin (MF20), desmin, cardiac troponin I, and sarcomeric {alpha}-actinin was positive. RT-PCR analysis showed that these differentiated cells express cardiac-specific genes. Transmission electron microscopy revealed a cardiomyocyte-like ultrastructure and typical sarcomers. These observations confirm that human umbilical cord-derived mesenchymal stemmore » cells can be chemically transformed into cardiomyocytes and can be considered as a source of cells for cellular cardiomyoplasty.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6]
  1. Department of Biochemistry, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)
  2. Department of Biochemistry, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of). E-mail: khatamibiochem@yahoo.com
  3. Department of Pathology, Zanjan Medical School, Zanjan (Iran, Islamic Republic of)
  4. National Cell Bank of Iran (NCBI), Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)
  5. Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modarres University, Tehran (Iran, Islamic Republic of)
  6. Department of Hematology, School of Medical Sciences, Tarbiat Modarres University, Tehran (Iran, Islamic Republic of)
Publication Date:
OSTI Identifier:
20798796
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 340; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.12.047; PII: S0006-291X(05)02804-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CONNECTIVE TISSUE CELLS; IN VITRO; MORPHOLOGICAL CHANGES; MYOCARDIUM; MYOSIN; POLYMERASE CHAIN REACTION; STEM CELLS; TRANSMISSION ELECTRON MICROSCOPY; VEINS

Citation Formats

Kadivar, Mehdi, Khatami, Shohreh, Mortazavi, Yousef, Shokrgozar, Mohammad Ali, Taghikhani, Mohammad, and Soleimani, Masoud. In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.12.047.
Kadivar, Mehdi, Khatami, Shohreh, Mortazavi, Yousef, Shokrgozar, Mohammad Ali, Taghikhani, Mohammad, & Soleimani, Masoud. In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells. United States. doi:10.1016/j.bbrc.2005.12.047.
Kadivar, Mehdi, Khatami, Shohreh, Mortazavi, Yousef, Shokrgozar, Mohammad Ali, Taghikhani, Mohammad, and Soleimani, Masoud. Fri . "In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells". United States. doi:10.1016/j.bbrc.2005.12.047.
@article{osti_20798796,
title = {In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells},
author = {Kadivar, Mehdi and Khatami, Shohreh and Mortazavi, Yousef and Shokrgozar, Mohammad Ali and Taghikhani, Mohammad and Soleimani, Masoud},
abstractNote = {Cardiomyocyte loss in the ischemically injured human heart often leads to irreversible defects in cardiac function. Recently, cellular cardiomyoplasty with mesenchymal stem cells, which are multipotent cells with the ability to differentiate into specialized cells under appropriate stimuli, has emerged as a new approach for repairing damaged myocardium. In the present study, the potential of human umbilical cord-derived mesenchymal stem cells to differentiate into cells with characteristics of cardiomyocyte was investigated. Mesenchymal stem cells were isolated from endothelial/subendothelial layers of the human umbilical cords using a method similar to that of human umbilical vein endothelial cell isolation. Isolated cells were characterized by transdifferentiation ability to adipocytes and osteoblasts, and also with flow cytometry analysis. After treatment with 5-azacytidine, the human umbilical cord-derived mesenchymal stem cells were morphologically transformed into cardiomyocyte-like cells and expressed cardiac differentiation markers. During the differentiation, cells were monitored by a phase contrast microscope and their morphological changes were demonstrated. Immunostaining of the differentiated cells for sarcomeric myosin (MF20), desmin, cardiac troponin I, and sarcomeric {alpha}-actinin was positive. RT-PCR analysis showed that these differentiated cells express cardiac-specific genes. Transmission electron microscopy revealed a cardiomyocyte-like ultrastructure and typical sarcomers. These observations confirm that human umbilical cord-derived mesenchymal stem cells can be chemically transformed into cardiomyocytes and can be considered as a source of cells for cellular cardiomyoplasty.},
doi = {10.1016/j.bbrc.2005.12.047},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 340,
place = {United States},
year = {Fri Feb 10 00:00:00 EST 2006},
month = {Fri Feb 10 00:00:00 EST 2006}
}