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Title: Perinuclear localisation of cellular retinoic acid binding protein I mRNA

Abstract

Retinoids are important metabolic and developmental regulators that act through nuclear receptors. The cellular retinoic acid binding protein CRABPI has been suggested to play a role in trafficking of retinoic acid but its exact functions and subcellular localisation remain unclear. Here we show that in CHO cells both exogenous CRABPI transcripts and tagged CRABPI protein have a perinuclear distribution that depends upon the 3'UTR of the mRNA. The CRABPI 3'UTR conferred perinuclear localisation on globin reporter transcripts. Deletion analysis indicated that First 123nt of CRABPI 3'UTR are necessary for localisation of both CRABPI mRNA and protein. We propose that CRABPI mRNA is localised by a signal within its 3'UTR and that this partly determines the distribution of CRABPI protein.

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle-upon-Tyne NE2 4HH (United Kingdom)
  2. Institute for Cell and Molecular Biosciences, University of Newcastle, Newcastle-upon-Tyne NE2 4HH (United Kingdom). E-mail: j.e.hesketh@ncl.ac.uk
Publication Date:
OSTI Identifier:
20798788
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 340; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.12.006; PII: S0006-291X(05)02732-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARBOXYLIC ACIDS; CHO CELLS; GLOBINS; MESSENGER-RNA; RECEPTORS; RETINOIC ACID; SIGNALS

Citation Formats

Levadoux-Martin, M., Li, Y., Blackburn, A., Chabanon, H., and Hesketh, J.E. Perinuclear localisation of cellular retinoic acid binding protein I mRNA. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.12.006.
Levadoux-Martin, M., Li, Y., Blackburn, A., Chabanon, H., & Hesketh, J.E. Perinuclear localisation of cellular retinoic acid binding protein I mRNA. United States. doi:10.1016/j.bbrc.2005.12.006.
Levadoux-Martin, M., Li, Y., Blackburn, A., Chabanon, H., and Hesketh, J.E. Fri . "Perinuclear localisation of cellular retinoic acid binding protein I mRNA". United States. doi:10.1016/j.bbrc.2005.12.006.
@article{osti_20798788,
title = {Perinuclear localisation of cellular retinoic acid binding protein I mRNA},
author = {Levadoux-Martin, M. and Li, Y. and Blackburn, A. and Chabanon, H. and Hesketh, J.E.},
abstractNote = {Retinoids are important metabolic and developmental regulators that act through nuclear receptors. The cellular retinoic acid binding protein CRABPI has been suggested to play a role in trafficking of retinoic acid but its exact functions and subcellular localisation remain unclear. Here we show that in CHO cells both exogenous CRABPI transcripts and tagged CRABPI protein have a perinuclear distribution that depends upon the 3'UTR of the mRNA. The CRABPI 3'UTR conferred perinuclear localisation on globin reporter transcripts. Deletion analysis indicated that First 123nt of CRABPI 3'UTR are necessary for localisation of both CRABPI mRNA and protein. We propose that CRABPI mRNA is localised by a signal within its 3'UTR and that this partly determines the distribution of CRABPI protein.},
doi = {10.1016/j.bbrc.2005.12.006},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 340,
place = {United States},
year = {Fri Feb 03 00:00:00 EST 2006},
month = {Fri Feb 03 00:00:00 EST 2006}
}