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Title: Identification of a novel male germ cell-specific gene TESF-1 in mice

Abstract

Mammalian spermatogenesis is precisely regulated by many germ cell-specific factors. In search for such a germ cell-specific factor, we have identified a novel mouse gene testis-specific factor 1 (TESF-1). Messenger RNA of TESF-1 was found only in the testis and its expression appeared to be regulated in a developmental manner. Further analysis demonstrated that the expression of TESF-1 was specifically in male germ cells, supported by the observation that we were not able to detect the TESF-1 mRNA from at/at homozygous mutant testes, which lack germ cells. The deduced amino acid sequence of TESF-1 contains a leucine-zipper motif, a potential nuclear localization signal, and two cAMP- and cGMP-dependent protein kinase phosphorylation sites. The green fluorescent protein (GFP)-tagged TESF-1 fusion protein was expressed in COS-7 cells and localized primarily in the nucleus. Taken together, these results indicate that TESF-1 is a novel male germ cell-specific gene, and its protein product may function as a nuclear factor involved in the regulation of spermatogenesis.

Authors:
 [1];  [1];  [1];  [2];  [3]
  1. Department of Biological Sciences, Marshall University, Huntington, WV (United States)
  2. Department of Anatomy and Pathology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV (United States)
  3. Department of Biological Sciences, Marshall University, Huntington, WV (United States). E-mail: zhu4@marshall.edu
Publication Date:
OSTI Identifier:
20798771
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 340; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.11.152; PII: S0006-291X(05)02669-0; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; AMINO ACID SEQUENCE; AMP; GENE REGULATION; GENES; GERM CELLS; LEUCINE; MESSENGER-RNA; MICE; MUTANTS; PHOSPHORYLATION; PROTEINS; SPERMATOGENESIS; TESTES

Citation Formats

Fan Jun, Graham, Matthew, Akabane, Hiroto, Richardson, Laura L., and Zhu Guozhang. Identification of a novel male germ cell-specific gene TESF-1 in mice. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.11.152.
Fan Jun, Graham, Matthew, Akabane, Hiroto, Richardson, Laura L., & Zhu Guozhang. Identification of a novel male germ cell-specific gene TESF-1 in mice. United States. doi:10.1016/j.bbrc.2005.11.152.
Fan Jun, Graham, Matthew, Akabane, Hiroto, Richardson, Laura L., and Zhu Guozhang. Fri . "Identification of a novel male germ cell-specific gene TESF-1 in mice". United States. doi:10.1016/j.bbrc.2005.11.152.
@article{osti_20798771,
title = {Identification of a novel male germ cell-specific gene TESF-1 in mice},
author = {Fan Jun and Graham, Matthew and Akabane, Hiroto and Richardson, Laura L. and Zhu Guozhang},
abstractNote = {Mammalian spermatogenesis is precisely regulated by many germ cell-specific factors. In search for such a germ cell-specific factor, we have identified a novel mouse gene testis-specific factor 1 (TESF-1). Messenger RNA of TESF-1 was found only in the testis and its expression appeared to be regulated in a developmental manner. Further analysis demonstrated that the expression of TESF-1 was specifically in male germ cells, supported by the observation that we were not able to detect the TESF-1 mRNA from at/at homozygous mutant testes, which lack germ cells. The deduced amino acid sequence of TESF-1 contains a leucine-zipper motif, a potential nuclear localization signal, and two cAMP- and cGMP-dependent protein kinase phosphorylation sites. The green fluorescent protein (GFP)-tagged TESF-1 fusion protein was expressed in COS-7 cells and localized primarily in the nucleus. Taken together, these results indicate that TESF-1 is a novel male germ cell-specific gene, and its protein product may function as a nuclear factor involved in the regulation of spermatogenesis.},
doi = {10.1016/j.bbrc.2005.11.152},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 340,
place = {United States},
year = {Fri Feb 03 00:00:00 EST 2006},
month = {Fri Feb 03 00:00:00 EST 2006}
}