skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Luteolin, a flavonoid, inhibits AP-1 activation by basophils

Abstract

Flavonoids including luteolin, apigenin, and fisetin are inhibitors of IL-4 synthesis and CD40 ligand expression by basophils. This study was done to search for compounds with greater inhibitory activity of IL-4 expression and to clarify the molecular mechanisms through which flavonoids inhibit their expression. Of the 37 flavonoids and related compounds examined, ayanin, luteolin, and apigenin were the strongest inhibitors of IL-4 production by purified basophils in response to anti-IgE antibody plus IL-3. Luteolin did not suppress Syk or Lyn phosphorylation in basophils, nor did suppress p54/46 SAPK/JNK, p38 MAPK, and p44/42 MAPK activation by a basophilic cell line, KU812 cells, stimulated with A23187 and PMA. However, luteolin did inhibit phosphorylation of c-Jun and DNA binding activity of AP-1 in nuclear lysates from stimulated KU812 cells. These results provide a fundamental structure of flavonoids for IL-4 inhibition and demonstrate a novel action of flavonoids that suppresses the activation of AP-1.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]; ; ;  [1]; ;  [2]; ;  [3];  [1];  [4]
  1. Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Medical School, Osaka 565-0871 (Japan)
  2. Kyoto Pharmaceutical University, Kyoto 607-8412 (Japan)
  3. Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871 (Japan)
  4. Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Medical School, Osaka 565-0871 (Japan). E-mail: ttanak@imed3.med.osaka-u.ac.jp
Publication Date:
OSTI Identifier:
20798770
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 340; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.11.157; PII: S0006-291X(05)02705-1; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; BASOPHILS; BIOSYNTHESIS; DNA; FLAVONOIDS; INHIBITION; LIGANDS; LYMPHOKINES; PHOSPHORYLATION

Citation Formats

Hirano, Toru, Higa, Shinji, Arimitsu, Junsuke, Naka, Tetsuji, Ogata, Atsushi, Shima, Yoshihito, Fujimoto, Minoru, Yamadori, Tomoki, Ohkawara, Tomoharu, Kuwabara, Yusuke, Kawai, Mari, Matsuda, Hisashi, Yoshikawa, Masayuki, Maezaki, Naoyoshi, Tanaka, Tetsuaki, Kawase, Ichiro, and Tanaka, Toshio. Luteolin, a flavonoid, inhibits AP-1 activation by basophils. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.11.157.
Hirano, Toru, Higa, Shinji, Arimitsu, Junsuke, Naka, Tetsuji, Ogata, Atsushi, Shima, Yoshihito, Fujimoto, Minoru, Yamadori, Tomoki, Ohkawara, Tomoharu, Kuwabara, Yusuke, Kawai, Mari, Matsuda, Hisashi, Yoshikawa, Masayuki, Maezaki, Naoyoshi, Tanaka, Tetsuaki, Kawase, Ichiro, & Tanaka, Toshio. Luteolin, a flavonoid, inhibits AP-1 activation by basophils. United States. doi:10.1016/j.bbrc.2005.11.157.
Hirano, Toru, Higa, Shinji, Arimitsu, Junsuke, Naka, Tetsuji, Ogata, Atsushi, Shima, Yoshihito, Fujimoto, Minoru, Yamadori, Tomoki, Ohkawara, Tomoharu, Kuwabara, Yusuke, Kawai, Mari, Matsuda, Hisashi, Yoshikawa, Masayuki, Maezaki, Naoyoshi, Tanaka, Tetsuaki, Kawase, Ichiro, and Tanaka, Toshio. Fri . "Luteolin, a flavonoid, inhibits AP-1 activation by basophils". United States. doi:10.1016/j.bbrc.2005.11.157.
@article{osti_20798770,
title = {Luteolin, a flavonoid, inhibits AP-1 activation by basophils},
author = {Hirano, Toru and Higa, Shinji and Arimitsu, Junsuke and Naka, Tetsuji and Ogata, Atsushi and Shima, Yoshihito and Fujimoto, Minoru and Yamadori, Tomoki and Ohkawara, Tomoharu and Kuwabara, Yusuke and Kawai, Mari and Matsuda, Hisashi and Yoshikawa, Masayuki and Maezaki, Naoyoshi and Tanaka, Tetsuaki and Kawase, Ichiro and Tanaka, Toshio},
abstractNote = {Flavonoids including luteolin, apigenin, and fisetin are inhibitors of IL-4 synthesis and CD40 ligand expression by basophils. This study was done to search for compounds with greater inhibitory activity of IL-4 expression and to clarify the molecular mechanisms through which flavonoids inhibit their expression. Of the 37 flavonoids and related compounds examined, ayanin, luteolin, and apigenin were the strongest inhibitors of IL-4 production by purified basophils in response to anti-IgE antibody plus IL-3. Luteolin did not suppress Syk or Lyn phosphorylation in basophils, nor did suppress p54/46 SAPK/JNK, p38 MAPK, and p44/42 MAPK activation by a basophilic cell line, KU812 cells, stimulated with A23187 and PMA. However, luteolin did inhibit phosphorylation of c-Jun and DNA binding activity of AP-1 in nuclear lysates from stimulated KU812 cells. These results provide a fundamental structure of flavonoids for IL-4 inhibition and demonstrate a novel action of flavonoids that suppresses the activation of AP-1.},
doi = {10.1016/j.bbrc.2005.11.157},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 340,
place = {United States},
year = {Fri Feb 03 00:00:00 EST 2006},
month = {Fri Feb 03 00:00:00 EST 2006}
}