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Title: Effects of perfluorinated amphiphiles on backward swimming in Paramecium caudatum

Abstract

PFOS and PFOA are ubiquitous contaminants in the environment. We investigated the effects of fluorochemicals on calcium currents in Paramecium caudatum using its behavioral changes. Negatively charged amphiphiles prolonged backward swimming (BWS) of Paramecium. PFOS significantly prolonged BWS, while PFOA was less potent (EC{sub 5}: 29.8 {+-} 4.1 and 424.1 {+-} 124.0 {mu}M, respectively). The BWS prolongation was blocked by cadmium, indicating that the cellular calcium conductance had been modified. The positively charged amphiphile FOSAPrTMA shortened BWS (EC{sub 5}: 19.1 {+-} 17.3). Nonionic amphiphiles did not affect BWS. The longer-chain perfluorinated carboxylates PFNA and PFDA were more potent than PFOA (EC{sub 5}: 98.7 {+-} 20.1 and 60.4 {+-} 10.1 {mu}M, respectively). However, 1,8-perfluorooctanedioic acid and 1,10-perfluorodecanedioic acid did not prolong BWS. The critical micelle concentration (CMC) and BWS prolongation for negatively charged amphiphiles showed a clear correlation (r {sup 2} = 0.8008, p < 0.001). In summary, several perfluorochemicals and PFOS and PFOA had similar effects in Paramecium, while chain length, CMC, and electric charge were major determinants of BWS duration.

Authors:
 [1];  [1];  [2];  [1];  [3]
  1. Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 6068501 (Japan)
  2. (Japan)
  3. Department of Health and Environmental Sciences, Kyoto University Graduate School of Medicine, Kyoto 6068501 (Japan). E-mail: koizumi@pbh.med.kyoto-u.ac.jp
Publication Date:
OSTI Identifier:
20798744
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 339; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.11.048; PII: S0006-291X(05)02578-7; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CADMIUM; CALCIUM; CARBOXYLATION; ELECTRIC CHARGES; EXERCISE; PARAMECIUM; STRUCTURE-ACTIVITY RELATIONSHIPS

Citation Formats

Matsubara, Eriko, Harada, Kouji, Research Fellow of the Japan Society for the Promotion of Science, Tokyo 1028472, Inoue, Kayoko, and Koizumi, Akio. Effects of perfluorinated amphiphiles on backward swimming in Paramecium caudatum. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.11.048.
Matsubara, Eriko, Harada, Kouji, Research Fellow of the Japan Society for the Promotion of Science, Tokyo 1028472, Inoue, Kayoko, & Koizumi, Akio. Effects of perfluorinated amphiphiles on backward swimming in Paramecium caudatum. United States. doi:10.1016/j.bbrc.2005.11.048.
Matsubara, Eriko, Harada, Kouji, Research Fellow of the Japan Society for the Promotion of Science, Tokyo 1028472, Inoue, Kayoko, and Koizumi, Akio. Fri . "Effects of perfluorinated amphiphiles on backward swimming in Paramecium caudatum". United States. doi:10.1016/j.bbrc.2005.11.048.
@article{osti_20798744,
title = {Effects of perfluorinated amphiphiles on backward swimming in Paramecium caudatum},
author = {Matsubara, Eriko and Harada, Kouji and Research Fellow of the Japan Society for the Promotion of Science, Tokyo 1028472 and Inoue, Kayoko and Koizumi, Akio},
abstractNote = {PFOS and PFOA are ubiquitous contaminants in the environment. We investigated the effects of fluorochemicals on calcium currents in Paramecium caudatum using its behavioral changes. Negatively charged amphiphiles prolonged backward swimming (BWS) of Paramecium. PFOS significantly prolonged BWS, while PFOA was less potent (EC{sub 5}: 29.8 {+-} 4.1 and 424.1 {+-} 124.0 {mu}M, respectively). The BWS prolongation was blocked by cadmium, indicating that the cellular calcium conductance had been modified. The positively charged amphiphile FOSAPrTMA shortened BWS (EC{sub 5}: 19.1 {+-} 17.3). Nonionic amphiphiles did not affect BWS. The longer-chain perfluorinated carboxylates PFNA and PFDA were more potent than PFOA (EC{sub 5}: 98.7 {+-} 20.1 and 60.4 {+-} 10.1 {mu}M, respectively). However, 1,8-perfluorooctanedioic acid and 1,10-perfluorodecanedioic acid did not prolong BWS. The critical micelle concentration (CMC) and BWS prolongation for negatively charged amphiphiles showed a clear correlation (r {sup 2} = 0.8008, p < 0.001). In summary, several perfluorochemicals and PFOS and PFOA had similar effects in Paramecium, while chain length, CMC, and electric charge were major determinants of BWS duration.},
doi = {10.1016/j.bbrc.2005.11.048},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 339,
place = {United States},
year = {Fri Jan 13 00:00:00 EST 2006},
month = {Fri Jan 13 00:00:00 EST 2006}
}