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Title: Transcriptional activity of Pax3 is co-activated by TAZ

Abstract

Pax3 is a transcription factor which functions in embryonic development and human diseases. In a yeast two-hybrid screen with full-length Pax3 as bait, we isolated a clone encoding transcriptional co-activator with PDZ-binding motif (TAZ) from an E10.5 mouse embryo cDNA library. Co-immunoprecipitation and nuclear co-localization of TAZ with Pax3 suggest that their association is functionally relevant. In situ hybridization revealed TAZ and Pax3 expression to partially overlap in the paraxial mesoderm, limb buds, and the neural tube. In C2C12 myoblast cells and NIH3T3 cells, TAZ enhanced the transcriptional activity of Pax3 on artificial and microphthalmia-associated transcription factor promoter-luciferase constructs, suggesting that TAZ can function as a co-activator of Pax3. Functional interaction between Pax3 and TAZ may provide a clue to clarifying the mechanism by which Pax3 serves as a transcriptional activator during embryogenesis.

Authors:
 [1];  [2];  [1];  [3];  [4];  [4];  [4];  [5];  [4];  [6]
  1. Department of Integrative Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto, Kumamoto 860-0811 (Japan)
  2. (United States)
  3. (Japan)
  4. Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan)
  5. Division of Cardiology, Department of Internal Medicine, and Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390-9148 (United States)
  6. Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 (Japan). E-mail: kuri-tky@umin.ac.jp
Publication Date:
OSTI Identifier:
20798741
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 339; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.10.214; PII: S0006-291X(05)02482-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; EMBRYOS; HYBRIDIZATION; IN-SITU HYBRIDIZATION; LIMBS; LUCIFERASE; MICE; ONTOGENESIS; TRANSCRIPTION; TRANSCRIPTION FACTORS; YEASTS

Citation Formats

Murakami, Masao, Division of Cardiology, Department of Internal Medicine, and Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390-9148, Tominaga, Junji, Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Makita, Ryosuke, Uchijima, Yasunobu, Kurihara, Yukiko, Nakagawa, Osamu, Asano, Tomoichiro, and Kurihara, Hiroki. Transcriptional activity of Pax3 is co-activated by TAZ. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2005.10.214.
Murakami, Masao, Division of Cardiology, Department of Internal Medicine, and Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390-9148, Tominaga, Junji, Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Makita, Ryosuke, Uchijima, Yasunobu, Kurihara, Yukiko, Nakagawa, Osamu, Asano, Tomoichiro, & Kurihara, Hiroki. Transcriptional activity of Pax3 is co-activated by TAZ. United States. doi:10.1016/j.bbrc.2005.10.214.
Murakami, Masao, Division of Cardiology, Department of Internal Medicine, and Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390-9148, Tominaga, Junji, Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Makita, Ryosuke, Uchijima, Yasunobu, Kurihara, Yukiko, Nakagawa, Osamu, Asano, Tomoichiro, and Kurihara, Hiroki. Fri . "Transcriptional activity of Pax3 is co-activated by TAZ". United States. doi:10.1016/j.bbrc.2005.10.214.
@article{osti_20798741,
title = {Transcriptional activity of Pax3 is co-activated by TAZ},
author = {Murakami, Masao and Division of Cardiology, Department of Internal Medicine, and Department of Molecular Biology, University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Blvd., Dallas, TX 75390-9148 and Tominaga, Junji and Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 and Makita, Ryosuke and Uchijima, Yasunobu and Kurihara, Yukiko and Nakagawa, Osamu and Asano, Tomoichiro and Kurihara, Hiroki},
abstractNote = {Pax3 is a transcription factor which functions in embryonic development and human diseases. In a yeast two-hybrid screen with full-length Pax3 as bait, we isolated a clone encoding transcriptional co-activator with PDZ-binding motif (TAZ) from an E10.5 mouse embryo cDNA library. Co-immunoprecipitation and nuclear co-localization of TAZ with Pax3 suggest that their association is functionally relevant. In situ hybridization revealed TAZ and Pax3 expression to partially overlap in the paraxial mesoderm, limb buds, and the neural tube. In C2C12 myoblast cells and NIH3T3 cells, TAZ enhanced the transcriptional activity of Pax3 on artificial and microphthalmia-associated transcription factor promoter-luciferase constructs, suggesting that TAZ can function as a co-activator of Pax3. Functional interaction between Pax3 and TAZ may provide a clue to clarifying the mechanism by which Pax3 serves as a transcriptional activator during embryogenesis.},
doi = {10.1016/j.bbrc.2005.10.214},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 339,
place = {United States},
year = {Fri Jan 13 00:00:00 EST 2006},
month = {Fri Jan 13 00:00:00 EST 2006}
}
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