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Title: V{delta}1 T cell receptor binds specifically to MHC I chain related A: Molecular and biochemical evidences

Abstract

Human MHC class I chain-related A (MICA) is a tumor-associated antigen that can be recognized by V{delta}1 subset of tumor-infiltrating {gamma}{delta} T cells. We previously reported that immobilized recombinant MICA protein could induce the proliferation of tumor-infiltrating V{delta}1 {gamma}{delta} T cells in vitro. But there has been no direct evidence showing the engagement of {gamma}{delta} T cell receptors (TCR) of the induced cells with MICA. In the current investigation, we show that MICA induces specific cytolytic activity of the expanded {gamma}{delta} T cells. We expressed the coupled V domains from the MICA-induced T cells as a single polypeptide chain V{delta}V{gamma} TCR ({gamma}{delta} scTCR). Such scTCR can specifically bind MICA of HeLa cells. Direct interaction of {gamma}{delta} scTCRs with in vitro expressed MICA was monitored using an IAsys biosensor. We found that the V{delta}1 scTCR can specifically bind to immobilized MICA molecule and MICA{alpha}1{alpha}2 domains are responsible for the binding reaction.

Authors:
 [1];  [1];  [1];  [1];  [2]
  1. Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005 (China)
  2. Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005 (China). E-mail: heweiimu@public.bta.net.cn
Publication Date:
OSTI Identifier:
20795857
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 339; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.10.198; PII: S0006-291X(05)02465-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTIGENS; CELL PROLIFERATION; HELA CELLS; IN VITRO; MICA; NEOPLASMS; POLYPEPTIDES; RECEPTORS; TOXICITY

Citation Formats

Zhao Jianqing, Huang Jie, Chen Hui, Cui Lianxian, and He Wei. V{delta}1 T cell receptor binds specifically to MHC I chain related A: Molecular and biochemical evidences. United States: N. p., 2006. Web. doi:10.1016/J.BBRC.2005.1.
Zhao Jianqing, Huang Jie, Chen Hui, Cui Lianxian, & He Wei. V{delta}1 T cell receptor binds specifically to MHC I chain related A: Molecular and biochemical evidences. United States. doi:10.1016/J.BBRC.2005.1.
Zhao Jianqing, Huang Jie, Chen Hui, Cui Lianxian, and He Wei. Fri . "V{delta}1 T cell receptor binds specifically to MHC I chain related A: Molecular and biochemical evidences". United States. doi:10.1016/J.BBRC.2005.1.
@article{osti_20795857,
title = {V{delta}1 T cell receptor binds specifically to MHC I chain related A: Molecular and biochemical evidences},
author = {Zhao Jianqing and Huang Jie and Chen Hui and Cui Lianxian and He Wei},
abstractNote = {Human MHC class I chain-related A (MICA) is a tumor-associated antigen that can be recognized by V{delta}1 subset of tumor-infiltrating {gamma}{delta} T cells. We previously reported that immobilized recombinant MICA protein could induce the proliferation of tumor-infiltrating V{delta}1 {gamma}{delta} T cells in vitro. But there has been no direct evidence showing the engagement of {gamma}{delta} T cell receptors (TCR) of the induced cells with MICA. In the current investigation, we show that MICA induces specific cytolytic activity of the expanded {gamma}{delta} T cells. We expressed the coupled V domains from the MICA-induced T cells as a single polypeptide chain V{delta}V{gamma} TCR ({gamma}{delta} scTCR). Such scTCR can specifically bind MICA of HeLa cells. Direct interaction of {gamma}{delta} scTCRs with in vitro expressed MICA was monitored using an IAsys biosensor. We found that the V{delta}1 scTCR can specifically bind to immobilized MICA molecule and MICA{alpha}1{alpha}2 domains are responsible for the binding reaction.},
doi = {10.1016/J.BBRC.2005.1},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 339,
place = {United States},
year = {Fri Jan 06 00:00:00 EST 2006},
month = {Fri Jan 06 00:00:00 EST 2006}
}