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Title: Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals

Abstract

Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I{sup -/-} mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I{sup -/-} than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I{sup -/-} mice. These results demonstrate that Prx I{sup -/-} mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.

Authors:
 [1];  [2];  [3];  [1];  [1];  [1];  [1];  [3];  [2];  [4]
  1. Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
  2. Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
  3. Functional and Regulatory Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
  4. Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan). E-mail: teishii@md.tsukuba.ac.jp
Publication Date:
OSTI Identifier:
20795856
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 339; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.10.192; PII: S0006-291X(05)02476-9; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALANINES; BIOLOGICAL STRESS; ELECTRON SPIN RESONANCE; IN VIVO; KIDNEYS; LIVER; MICE; NITROXYL RADICALS; NTA; PARAMAGNETISM; PEROXIDASES

Citation Formats

Uwayama, Junya, Hirayama, Aki, Yanagawa, Toru, Warabi, Eiji, Sugimoto, Rika, Itoh, Ken, Yamamoto, Masayuki, Yoshida, Hiroshi, Koyama, Akio, and Ishii, Tetsuro. Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals. United States: N. p., 2006. Web. doi:10.1016/J.BBRC.2005.1.
Uwayama, Junya, Hirayama, Aki, Yanagawa, Toru, Warabi, Eiji, Sugimoto, Rika, Itoh, Ken, Yamamoto, Masayuki, Yoshida, Hiroshi, Koyama, Akio, & Ishii, Tetsuro. Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals. United States. doi:10.1016/J.BBRC.2005.1.
Uwayama, Junya, Hirayama, Aki, Yanagawa, Toru, Warabi, Eiji, Sugimoto, Rika, Itoh, Ken, Yamamoto, Masayuki, Yoshida, Hiroshi, Koyama, Akio, and Ishii, Tetsuro. Fri . "Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals". United States. doi:10.1016/J.BBRC.2005.1.
@article{osti_20795856,
title = {Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals},
author = {Uwayama, Junya and Hirayama, Aki and Yanagawa, Toru and Warabi, Eiji and Sugimoto, Rika and Itoh, Ken and Yamamoto, Masayuki and Yoshida, Hiroshi and Koyama, Akio and Ishii, Tetsuro},
abstractNote = {Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I{sup -/-} mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I{sup -/-} than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I{sup -/-} mice. These results demonstrate that Prx I{sup -/-} mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.},
doi = {10.1016/J.BBRC.2005.1},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 339,
place = {United States},
year = {Fri Jan 06 00:00:00 EST 2006},
month = {Fri Jan 06 00:00:00 EST 2006}
}
  • A study was carried out on the kinetics of thermal bimolecular reactions leading to the reduction of nitroxyl radicals by hydrazo compounds in a nematic liquid crystal. A positive deviation in the experimental reaction rate constant from the Arrhenius curve obtained in the isotropic phase at higher temperatures is found in the pretransition region of the isotropic phase and in the meso phase. A possible mechanism for the effect of the meso phase on the activation energy of the reactions studied was examined. A method was proposed for describing the temperature dependence of the experimental rate constant in the nematicmore » phase and an equation was obtained relating the rate constant with the order parameter of the liquid crystal and other physical parameters of the solventreagent system.« less
  • The Electron spin resonance studies on the reduction process of nitroxyl spin probes were carried out for 1mM {sup 14}N labeled nitroxyl radicals in pure water and 1 mM concentration of ascorbic acid as a function of time. The electron spin resonance parameters such as signal intensity ratio, line width, g-value, hyperfine coupling constant and rotational correlation time were determined. The half life time was estimated for 1mM {sup 14}N labeled nitroxyl radicals in 1 mM concentration of ascorbic acid. The ESR study reveals that the TEMPONE has narrowest line width and fast tumbling motion compared with TEMPO and TEMPOL.more » From the results, TEMPONE has long half life time and high stability compared with TEMPO and TEMPOL radical. Therefore, this study reveals that the TEMPONE radical can act as a good redox sensitive spin probe for molecular imaging.« less
  • The radioprotective activity of a number of biological amines, disulfide compounds and thiols at various concentrations was tested in an in vitro system, based on the cloning technique devised by Puck et al. It was found that none of the biological amines (histamine, adrenaline, noradrenaline, and serotonin) or disulfide compounds (cystamine and cystine) showed a protective activity. A definitive protection on the other hand was observed with the thiol compounds (cysteamine, cysteine, and AET). The protective activity of the latter compounds depended on the concentration which was used. Concentrations of 2 to 4 mm gave dose-reduction factors (DRF) between 1more » and 2. At higher concentrations (cysteamine 32 mm and cysteine 128 mm) DRF's approaching 4 were obtained, The best protection with AET was observed at a concentration of 128 mm (DRF 2.2) The protection by AET was meliorated if higher serum concentrations in the culture medium were used. The best protection on a mm basis was provided by cysteamine. None of the compounds protected when applied after irradiation. (auth)« less