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Title: Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals

Abstract

Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I{sup -/-} mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I{sup -/-} than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I{sup -/-} mice. These results demonstrate that Prx I{sup -/-} mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.

Authors:
 [1];  [2];  [3];  [1];  [1];  [1];  [1];  [3];  [2];  [4]
  1. Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
  2. Medical Sciences for Control of Pathological Processes, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
  3. Functional and Regulatory Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan)
  4. Biomolecular and Integrated Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki (Japan). E-mail: teishii@md.tsukuba.ac.jp
Publication Date:
OSTI Identifier:
20795856
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 339; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.10.192; PII: S0006-291X(05)02476-9; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ALANINES; BIOLOGICAL STRESS; ELECTRON SPIN RESONANCE; IN VIVO; KIDNEYS; LIVER; MICE; NITROXYL RADICALS; NTA; PARAMAGNETISM; PEROXIDASES

Citation Formats

Uwayama, Junya, Hirayama, Aki, Yanagawa, Toru, Warabi, Eiji, Sugimoto, Rika, Itoh, Ken, Yamamoto, Masayuki, Yoshida, Hiroshi, Koyama, Akio, and Ishii, Tetsuro. Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals. United States: N. p., 2006. Web. doi:10.1016/J.BBRC.2005.1.
Uwayama, Junya, Hirayama, Aki, Yanagawa, Toru, Warabi, Eiji, Sugimoto, Rika, Itoh, Ken, Yamamoto, Masayuki, Yoshida, Hiroshi, Koyama, Akio, & Ishii, Tetsuro. Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals. United States. doi:10.1016/J.BBRC.2005.1.
Uwayama, Junya, Hirayama, Aki, Yanagawa, Toru, Warabi, Eiji, Sugimoto, Rika, Itoh, Ken, Yamamoto, Masayuki, Yoshida, Hiroshi, Koyama, Akio, and Ishii, Tetsuro. Fri . "Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals". United States. doi:10.1016/J.BBRC.2005.1.
@article{osti_20795856,
title = {Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals},
author = {Uwayama, Junya and Hirayama, Aki and Yanagawa, Toru and Warabi, Eiji and Sugimoto, Rika and Itoh, Ken and Yamamoto, Masayuki and Yoshida, Hiroshi and Koyama, Akio and Ishii, Tetsuro},
abstractNote = {Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I{sup -/-} mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I{sup -/-} than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I{sup -/-} mice. These results demonstrate that Prx I{sup -/-} mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.},
doi = {10.1016/J.BBRC.2005.1},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 339,
place = {United States},
year = {Fri Jan 06 00:00:00 EST 2006},
month = {Fri Jan 06 00:00:00 EST 2006}
}