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Title: Differentiation-inducing factor-1 suppresses gene expression of cyclin D1 in tumor cells

Abstract

To determine the mechanism by which differentiation-inducing factor-1 (DIF-1), a morphogen of Dictyostelium discoideum, inhibits tumor cell proliferation, we examined the effect of DIF-1 on the gene expression of cyclin D1. DIF-1 strongly reduced the expression of cyclin D1 mRNA and correspondingly decreased the amount of {beta}-catenin in HeLa cells and squamous cell carcinoma cells. DIF-1 activated glycogen synthase kinase-3{beta} (GSK-3{beta}) and inhibition of GSK-3{beta} attenuated the DIF-1-induced {beta}-catenin degradation, indicating the involvement of GSK-3{beta} in this effect. Moreover, DIF-1 reduced the activities of T-cell factor (TCF)/lymphoid enhancer factor (LEF) reporter plasmid and a reporter gene driven by the human cyclin D1 promoter. Eliminating the TCF/LEF consensus site from the cyclin D1 promoter diminished the effect of DIF-1. These results suggest that DIF-1 inhibits Wnt/{beta}-catenin signaling, resulting in the suppression of cyclin D1 promoter activity.

Authors:
 [1];  [2];  [1];  [1];  [3];  [4];  [1];  [1]
  1. Department of Clinical Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582 (Japan)
  2. Department of Clinical Pharmacology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582 (Japan). E-mail: yanaga@clipharm.med.kyushu-u.ac.jp
  3. Department of Molecular and Cellular Biochemistry, Graduate School of Dental Sciences, Kyushu University, Fukuoka 812-8582 (Japan)
  4. Department of Applied Chemistry, Faculty of Engineering, Ehime University, Matsuyama 790-8577 (Japan)
Publication Date:
OSTI Identifier:
20793218
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 338; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.10.018; PII: S0006-291X(05)02264-3; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CARCINOMAS; CELL PROLIFERATION; GENES; GLYCOGEN; HELA CELLS; INHIBITION; PROMOTERS

Citation Formats

Yasmin, Tania, Takahashi-Yanaga, Fumi, Mori, Jun, Miwa, Yoshikazu, Hirata, Masato, Watanabe, Yutaka, Morimoto, Sachio, and Sasaguri, Toshiyuki. Differentiation-inducing factor-1 suppresses gene expression of cyclin D1 in tumor cells. United States: N. p., 2005. Web. doi:10.1016/J.BBRC.2005.1.
Yasmin, Tania, Takahashi-Yanaga, Fumi, Mori, Jun, Miwa, Yoshikazu, Hirata, Masato, Watanabe, Yutaka, Morimoto, Sachio, & Sasaguri, Toshiyuki. Differentiation-inducing factor-1 suppresses gene expression of cyclin D1 in tumor cells. United States. doi:10.1016/J.BBRC.2005.1.
Yasmin, Tania, Takahashi-Yanaga, Fumi, Mori, Jun, Miwa, Yoshikazu, Hirata, Masato, Watanabe, Yutaka, Morimoto, Sachio, and Sasaguri, Toshiyuki. Fri . "Differentiation-inducing factor-1 suppresses gene expression of cyclin D1 in tumor cells". United States. doi:10.1016/J.BBRC.2005.1.
@article{osti_20793218,
title = {Differentiation-inducing factor-1 suppresses gene expression of cyclin D1 in tumor cells},
author = {Yasmin, Tania and Takahashi-Yanaga, Fumi and Mori, Jun and Miwa, Yoshikazu and Hirata, Masato and Watanabe, Yutaka and Morimoto, Sachio and Sasaguri, Toshiyuki},
abstractNote = {To determine the mechanism by which differentiation-inducing factor-1 (DIF-1), a morphogen of Dictyostelium discoideum, inhibits tumor cell proliferation, we examined the effect of DIF-1 on the gene expression of cyclin D1. DIF-1 strongly reduced the expression of cyclin D1 mRNA and correspondingly decreased the amount of {beta}-catenin in HeLa cells and squamous cell carcinoma cells. DIF-1 activated glycogen synthase kinase-3{beta} (GSK-3{beta}) and inhibition of GSK-3{beta} attenuated the DIF-1-induced {beta}-catenin degradation, indicating the involvement of GSK-3{beta} in this effect. Moreover, DIF-1 reduced the activities of T-cell factor (TCF)/lymphoid enhancer factor (LEF) reporter plasmid and a reporter gene driven by the human cyclin D1 promoter. Eliminating the TCF/LEF consensus site from the cyclin D1 promoter diminished the effect of DIF-1. These results suggest that DIF-1 inhibits Wnt/{beta}-catenin signaling, resulting in the suppression of cyclin D1 promoter activity.},
doi = {10.1016/J.BBRC.2005.1},
journal = {Biochemical and Biophysical Research Communications},
number = 2,
volume = 338,
place = {United States},
year = {Fri Dec 16 00:00:00 EST 2005},
month = {Fri Dec 16 00:00:00 EST 2005}
}