Simultaneous determination of size and refractive index of red blood cells by light scattering measurements

Journal Article · Mon Feb 20 00:00:00 EST 2006 · Applied Physics Letters

DOI:https://doi.org/10.1063/1.2176854· OSTI ID:20778694

Ghosh, N; Buddhiwant, P; Uppal, A; Majumder, S K; Patel, H S; Gupta, P K ^[1]

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1. Biomedical Applications Section, Centre for Advanced Technology, Indore, 452013 (India)

We present a fast and accurate approach for simultaneous determination of both the mean diameter and refractive index of a collection of red blood cells (RBCs). The approach uses the peak frequency of the power spectrum and the corresponding phase angle obtained by performing Fourier transform on the measured angular distribution of scattered light to determine these parameters. Results on the measurement of two important clinical parameters, the mean cell volume and mean cell hemoglobin concentration of a collection of RBCs, are presented.

• OSTI ID: 20778694
• Journal Information: Applied Physics Letters, Vol. 88, Issue 8; Other Information: DOI: 10.1063/1.2176854; (c) 2006 American Institute of Physics; Country of input: International Atomic Energy Agency (IAEA); ISSN 0003-6951
• Country of Publication: United States
• Language: English

Related Subjects

71 CLASSICAL AND QUANTUM MECHANICS
GENERAL PHYSICS
ANGULAR DISTRIBUTION
BIOPHYSICS
BLOOD CELLS
DIAGNOSIS
FOURIER TRANSFORMATION
HEMOGLOBIN
LIGHT SCATTERING
PATIENTS
REFRACTIVE INDEX