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Title: Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation

Abstract

The retinoblastoma protein-interacting zinc-finger (RIZ) gene, a member of the nuclear protein methyltransferase superfamily, is characterized by the presence of the N-terminal PR domain. The RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the PR (PRDI-BF1 and RIZ homologous) domain, RIZ2 lacks it. RIZ gene expression is altered in a variety of human cancers and RIZ1 is now considered to be a candidate tumor suppressor. Estradiol treatment of MCF-7 cells produced a selective decrease of RIZ1 transcript and an increase of total RIZ mRNA. Experiments of chromatin immunoprecipitation indicated that RIZ2 protein expression was controlled by estrogen receptor and RIZ1 had a direct repressor function on c-myc gene expression. To investigate the role of RIZ gene products as regulators of the proliferation/differentiation transition, we analyzed the effects of forced suppression of RIZ1 induced in MCF-7 cells by siRNA of the PR domain-containing form. Silencing of RIZ1 expression stimulated cell proliferation, similar to the effect of estradiol on these cells, associated with a transient increase of c-myc expression.

Authors:
 [1];  [2];  [2];  [2];  [2];  [2];  [2]
  1. Dipartimento di Patologia generale, Seconda Universita degli studi di Napoli, Via Luigi de Crecchio 7, I-80138 Naples (Italy). E-mail: moncharmont@unimol.it
  2. Dipartimento di Patologia generale, Seconda Universita degli studi di Napoli, Via Luigi de Crecchio 7, I-80138 Naples (Italy)
Publication Date:
OSTI Identifier:
20775336
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; Journal Volume: 312; Journal Issue: 3; Other Information: DOI: 10.1016/j.yexcr.2005.11.002; PII: S0014-4827(05)00523-9; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL PROLIFERATION; CHROMATIN; ESTRADIOL; GENE REGULATION; GENES; INHIBITION; MAMMARY GLANDS; MODULATION; NEOPLASMS; RECEPTORS; ZINC

Citation Formats

Gazzerro, Patrizia, Abbondanza, Ciro, D'Arcangelo, Andrea, Rossi, Mariangela, Medici, Nicola, Moncharmont, Bruno, and Puca, Giovanni Alfredo. Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation. United States: N. p., 2006. Web. doi:10.1016/j.yexcr.2005.11.002.
Gazzerro, Patrizia, Abbondanza, Ciro, D'Arcangelo, Andrea, Rossi, Mariangela, Medici, Nicola, Moncharmont, Bruno, & Puca, Giovanni Alfredo. Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation. United States. doi:10.1016/j.yexcr.2005.11.002.
Gazzerro, Patrizia, Abbondanza, Ciro, D'Arcangelo, Andrea, Rossi, Mariangela, Medici, Nicola, Moncharmont, Bruno, and Puca, Giovanni Alfredo. Wed . "Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation". United States. doi:10.1016/j.yexcr.2005.11.002.
@article{osti_20775336,
title = {Modulation of RIZ gene expression is associated to estradiol control of MCF-7 breast cancer cell proliferation},
author = {Gazzerro, Patrizia and Abbondanza, Ciro and D'Arcangelo, Andrea and Rossi, Mariangela and Medici, Nicola and Moncharmont, Bruno and Puca, Giovanni Alfredo},
abstractNote = {The retinoblastoma protein-interacting zinc-finger (RIZ) gene, a member of the nuclear protein methyltransferase superfamily, is characterized by the presence of the N-terminal PR domain. The RIZ gene encodes for two proteins, RIZ1 and RIZ2. While RIZ1 contains the PR (PRDI-BF1 and RIZ homologous) domain, RIZ2 lacks it. RIZ gene expression is altered in a variety of human cancers and RIZ1 is now considered to be a candidate tumor suppressor. Estradiol treatment of MCF-7 cells produced a selective decrease of RIZ1 transcript and an increase of total RIZ mRNA. Experiments of chromatin immunoprecipitation indicated that RIZ2 protein expression was controlled by estrogen receptor and RIZ1 had a direct repressor function on c-myc gene expression. To investigate the role of RIZ gene products as regulators of the proliferation/differentiation transition, we analyzed the effects of forced suppression of RIZ1 induced in MCF-7 cells by siRNA of the PR domain-containing form. Silencing of RIZ1 expression stimulated cell proliferation, similar to the effect of estradiol on these cells, associated with a transient increase of c-myc expression.},
doi = {10.1016/j.yexcr.2005.11.002},
journal = {Experimental Cell Research},
number = 3,
volume = 312,
place = {United States},
year = {Wed Feb 01 00:00:00 EST 2006},
month = {Wed Feb 01 00:00:00 EST 2006}
}