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Title: Reference range levels of polycyclic aromatic hydrocarbons in the US population by measurement of urinary monohydroxy metabolites

Abstract

We developed a gas chromatography isotope-dilution high-resolution mass spectrometry (GC/Id-HRMS) method for measuring 14 polycyclic aromatic hydrocarbon (PAH) metabolites representing seven parent PAHs in 3 mL of urine at low parts-per-trillion levels. PAH levels were determined in urine samples collected in 1999 and 2000 from approximately 2400 participants in the National Health and Nutrition Examination Survey, and, for the first time, reference range values were calculated for these metabolites in the US population. Using this GC/ID-HRMS method, we found detectable concentrations for monohydroxy metabolite isomers of fluorene, phenanthrene, fluoranthene, pyrene, and chrysene, benzo[c]phenanthrene, and benz[a]anthracene. Some monohydroxy metabolite isomers of benzo[c]phenanthrene, chrysene, and benz[a]anthracene exhibited low detection frequencies that did not allow for geometric mean calculations. Our study results enabled us to establish a reference range for the targeted PAHs in the general US population.

Authors:
 [1];  [2];  [2];  [2];  [2];  [2];  [2];  [2];  [2]
  1. Division of Environment Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341 (United States). E-mail: jag2@cdc.gov
  2. Division of Environment Health Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Atlanta, GA 30341 (United States)
Publication Date:
OSTI Identifier:
20775308
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Research; Journal Volume: 100; Journal Issue: 3; Other Information: DOI: 10.1016/j.envres.2005.06.004; PII: S0013-9351(05)00087-3; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANTHRACENE; BIOLOGICAL MARKERS; CHRYSENE; FLUORENE; GAS CHROMATOGRAPHY; ISOMERS; ISOTOPE DILUTION; MASS SPECTROSCOPY; METABOLITES; NUTRITION; PHENANTHRENE; POLYCYCLIC AROMATIC HYDROCARBONS; PYRENE; URINE

Citation Formats

Grainger, James, Huang, Wenlin, Patterson, Donald G., Turner, Wayman E., Pirkle, James, Caudill, Samuel P., Wang, Richard Y., Needham, Larry L., and Sampson, Eric J.. Reference range levels of polycyclic aromatic hydrocarbons in the US population by measurement of urinary monohydroxy metabolites. United States: N. p., 2006. Web. doi:10.1016/j.envres.2005.06.004.
Grainger, James, Huang, Wenlin, Patterson, Donald G., Turner, Wayman E., Pirkle, James, Caudill, Samuel P., Wang, Richard Y., Needham, Larry L., & Sampson, Eric J.. Reference range levels of polycyclic aromatic hydrocarbons in the US population by measurement of urinary monohydroxy metabolites. United States. doi:10.1016/j.envres.2005.06.004.
Grainger, James, Huang, Wenlin, Patterson, Donald G., Turner, Wayman E., Pirkle, James, Caudill, Samuel P., Wang, Richard Y., Needham, Larry L., and Sampson, Eric J.. Wed . "Reference range levels of polycyclic aromatic hydrocarbons in the US population by measurement of urinary monohydroxy metabolites". United States. doi:10.1016/j.envres.2005.06.004.
@article{osti_20775308,
title = {Reference range levels of polycyclic aromatic hydrocarbons in the US population by measurement of urinary monohydroxy metabolites},
author = {Grainger, James and Huang, Wenlin and Patterson, Donald G. and Turner, Wayman E. and Pirkle, James and Caudill, Samuel P. and Wang, Richard Y. and Needham, Larry L. and Sampson, Eric J.},
abstractNote = {We developed a gas chromatography isotope-dilution high-resolution mass spectrometry (GC/Id-HRMS) method for measuring 14 polycyclic aromatic hydrocarbon (PAH) metabolites representing seven parent PAHs in 3 mL of urine at low parts-per-trillion levels. PAH levels were determined in urine samples collected in 1999 and 2000 from approximately 2400 participants in the National Health and Nutrition Examination Survey, and, for the first time, reference range values were calculated for these metabolites in the US population. Using this GC/ID-HRMS method, we found detectable concentrations for monohydroxy metabolite isomers of fluorene, phenanthrene, fluoranthene, pyrene, and chrysene, benzo[c]phenanthrene, and benz[a]anthracene. Some monohydroxy metabolite isomers of benzo[c]phenanthrene, chrysene, and benz[a]anthracene exhibited low detection frequencies that did not allow for geometric mean calculations. Our study results enabled us to establish a reference range for the targeted PAHs in the general US population.},
doi = {10.1016/j.envres.2005.06.004},
journal = {Environmental Research},
number = 3,
volume = 100,
place = {United States},
year = {Wed Mar 15 00:00:00 EST 2006},
month = {Wed Mar 15 00:00:00 EST 2006}
}