Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059

Journal Article · Thu Sep 15 00:00:00 EDT 2005 · Virology

DOI:https://doi.org/10.1016/j.virol.2005.06.015· OSTI ID:20729129

Murata, Takayuki ^[1]; Hijikata, Makoto ^[1]; Shimotohno, Kunitada ^[1]

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1. Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507 (Japan)

Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IRES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication.

• OSTI ID: 20729129
• Journal Information: Virology, Vol. 340, Issue 1; Other Information: DOI: 10.1016/j.virol.2005.06.015; PII: S0042-6822(05)00354-5; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
BIOSYNTHESIS
INCUBATION
INFECTIOUS HEPATITIS
PROTEINS
RNA
VIRUSES