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Title: Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection

Abstract

Previously, we found an up-regulation of tumor necrosis factor alpha (TNF)-{alpha} and an imbalance of TNF receptors in sheep experimentally infected with bovine leukemia virus (BLV). In order to investigate the different TNF-{alpha}-induced responses, in this study we examined the TNF-{alpha}-induced proliferative response and the expression levels of two distinct TNF receptors on peripheral blood mononuclear cells (PBMC) derived from BLV-uninfected cattle and BLV-infected cattle that were aleukemic (AL) or had persistent lymphocytosis (PL). The proliferative response of PBMC isolated from those cattle with PL in the presence of recombinant bovine TNF-{alpha} (rTNF-{alpha}) was significantly higher than those from AL cattle and uninfected cattle and the cells from PL cattle expressed significantly higher mRNA levels of TNF receptor type II (TNF-RII) than those from AL and BLV-uninfected cattle. No difference was found in TNF-RI mRNA levels. Most cells expressing TNF-RII in PL cattle were CD5{sup +} or sIgM{sup +} cells and these cells showed resistance to TNF-{alpha}-induced apoptosis. Additionally, there were significant positive correlations between the changes in provirus load and TNF-RII mRNA levels, and TNF-{alpha}-induced proliferation and TNF-RII mRNA levels. These data suggest that imbalance in the expression of TNF receptors could at least in part contribute to themore » progression of lymphocytosis in BLV infection.« less

Authors:
 [1];  [1];  [2];  [3];  [2];  [2];  [1];  [1]
  1. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818 (Japan)
  2. Faculty of Agriculture, Iwate University, Morioka, Iwate 020-8550 (Japan)
  3. Hokkaido Animal Research Center, Shintoku, Hokkaido 081-0038 (Japan)
Publication Date:
OSTI Identifier:
20729124
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 339; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2005.06.010; PII: S0042-6822(05)00338-7; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; APOPTOSIS; BLOOD; CATTLE; CELL PROLIFERATION; GENE REGULATION; LEUKEMIA VIRUSES; RECEPTORS; SHEEP

Citation Formats

Konnai, Satoru, Usui, Tatsufumi, Ikeda, Manabu, Kohara, Junko, Hirata, Toh-ichi, Okada, Kosuke, Ohashi, Kazuhiko, and Onuma, Misao. Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection. United States: N. p., 2005. Web. doi:10.1016/j.virol.2005.06.010.
Konnai, Satoru, Usui, Tatsufumi, Ikeda, Manabu, Kohara, Junko, Hirata, Toh-ichi, Okada, Kosuke, Ohashi, Kazuhiko, & Onuma, Misao. Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection. United States. https://doi.org/10.1016/j.virol.2005.06.010
Konnai, Satoru, Usui, Tatsufumi, Ikeda, Manabu, Kohara, Junko, Hirata, Toh-ichi, Okada, Kosuke, Ohashi, Kazuhiko, and Onuma, Misao. 2005. "Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection". United States. https://doi.org/10.1016/j.virol.2005.06.010.
@article{osti_20729124,
title = {Imbalance of tumor necrosis factor receptors during progression in bovine leukemia virus infection},
author = {Konnai, Satoru and Usui, Tatsufumi and Ikeda, Manabu and Kohara, Junko and Hirata, Toh-ichi and Okada, Kosuke and Ohashi, Kazuhiko and Onuma, Misao},
abstractNote = {Previously, we found an up-regulation of tumor necrosis factor alpha (TNF)-{alpha} and an imbalance of TNF receptors in sheep experimentally infected with bovine leukemia virus (BLV). In order to investigate the different TNF-{alpha}-induced responses, in this study we examined the TNF-{alpha}-induced proliferative response and the expression levels of two distinct TNF receptors on peripheral blood mononuclear cells (PBMC) derived from BLV-uninfected cattle and BLV-infected cattle that were aleukemic (AL) or had persistent lymphocytosis (PL). The proliferative response of PBMC isolated from those cattle with PL in the presence of recombinant bovine TNF-{alpha} (rTNF-{alpha}) was significantly higher than those from AL cattle and uninfected cattle and the cells from PL cattle expressed significantly higher mRNA levels of TNF receptor type II (TNF-RII) than those from AL and BLV-uninfected cattle. No difference was found in TNF-RI mRNA levels. Most cells expressing TNF-RII in PL cattle were CD5{sup +} or sIgM{sup +} cells and these cells showed resistance to TNF-{alpha}-induced apoptosis. Additionally, there were significant positive correlations between the changes in provirus load and TNF-RII mRNA levels, and TNF-{alpha}-induced proliferation and TNF-RII mRNA levels. These data suggest that imbalance in the expression of TNF receptors could at least in part contribute to the progression of lymphocytosis in BLV infection.},
doi = {10.1016/j.virol.2005.06.010},
url = {https://www.osti.gov/biblio/20729124}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 339,
place = {United States},
year = {Thu Sep 01 00:00:00 EDT 2005},
month = {Thu Sep 01 00:00:00 EDT 2005}
}