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Title: Host range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain

Abstract

Vaccinia Tian Tan (VTT) was used as a vaccine against smallpox in China for millions of people before 1980, yet the biological characteristics of the virus remain unclear. We have characterized VTT with respect to its host cell range, growth properties in vitro, and virulence in vivo. We found that 11 of the 12 mammalian cell lines studied are permissive to VTT infection whereas one, CHO-K1, is non-permissive. Using electron microscopy and sequence analysis, we found that the restriction of VTT replication in CHO-K1 is at a step before viral maturation probably due to the loss of the V025 gene. Moreover, VTT is significantly less virulent than vaccinia WR but remains neurovirulent in mice and causes significant body weight loss after intranasal inoculation. Our data demonstrate the need for further attenuation of VTT to serve either as a safer smallpox vaccine or as a live vaccine vector for other pathogens.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [2]
  1. Modern Virology Research Center and AIDS Center, National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei 430072 (China)
  2. Modern Virology Research Center and AIDS Center, National Key Laboratory of Virology, College of Life Sciences, Wuhan University, Hubei 430072 (China) and Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016 (United States)
Publication Date:
OSTI Identifier:
20726024
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 335; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2005.02.014; PII: S0042-6822(05)00119-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ATTENUATION; ELECTRON MICROSCOPY; GROWTH; IN VITRO; IN VIVO; INOCULATION; MATURATION; MICE; PATHOGENS; STRUCTURAL CHEMICAL ANALYSIS; VACCINES; VACCINIA VIRUS; VIRULENCE

Citation Formats

Qing, Fang, Lin, Yang, Weijun, Zhu, Li, Liu, Haibo, Wang, Wenbo, Yu, Genfu, Xiao, Po, Tien, Linqi, Zhang, Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016, AIDS Research Center, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, and Zhiwei, Chen. Host range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain. United States: N. p., 2005. Web. doi:10.1016/j.virol.2005.02.014.
Qing, Fang, Lin, Yang, Weijun, Zhu, Li, Liu, Haibo, Wang, Wenbo, Yu, Genfu, Xiao, Po, Tien, Linqi, Zhang, Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016, AIDS Research Center, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, & Zhiwei, Chen. Host range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain. United States. https://doi.org/10.1016/j.virol.2005.02.014
Qing, Fang, Lin, Yang, Weijun, Zhu, Li, Liu, Haibo, Wang, Wenbo, Yu, Genfu, Xiao, Po, Tien, Linqi, Zhang, Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016, AIDS Research Center, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, and Zhiwei, Chen. Tue . "Host range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain". United States. https://doi.org/10.1016/j.virol.2005.02.014.
@article{osti_20726024,
title = {Host range, growth property, and virulence of the smallpox vaccine: Vaccinia virus Tian Tan strain},
author = {Qing, Fang and Lin, Yang and Weijun, Zhu and Li, Liu and Haibo, Wang and Wenbo, Yu and Genfu, Xiao and Po, Tien and Linqi, Zhang and Aaron Diamond AIDS Research Center, Rockefeller University, New York, NY 10016 and AIDS Research Center, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing and Zhiwei, Chen},
abstractNote = {Vaccinia Tian Tan (VTT) was used as a vaccine against smallpox in China for millions of people before 1980, yet the biological characteristics of the virus remain unclear. We have characterized VTT with respect to its host cell range, growth properties in vitro, and virulence in vivo. We found that 11 of the 12 mammalian cell lines studied are permissive to VTT infection whereas one, CHO-K1, is non-permissive. Using electron microscopy and sequence analysis, we found that the restriction of VTT replication in CHO-K1 is at a step before viral maturation probably due to the loss of the V025 gene. Moreover, VTT is significantly less virulent than vaccinia WR but remains neurovirulent in mice and causes significant body weight loss after intranasal inoculation. Our data demonstrate the need for further attenuation of VTT to serve either as a safer smallpox vaccine or as a live vaccine vector for other pathogens.},
doi = {10.1016/j.virol.2005.02.014},
url = {https://www.osti.gov/biblio/20726024}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 335,
place = {United States},
year = {2005},
month = {5}
}