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Title: Equine infectious anemia virus-infected dendritic cells retain antigen presentation capability

Abstract

To determine if equine monocyte-derived dendritic cells (DC) were susceptible to equine infectious anemia virus (EIAV) infection, ex vivo-generated DC were infected with virus in vitro. EIAV antigen was detected by immunofluorescence 3 days post-infection with maximum antigen being detected on day 4, whereas there was no antigen detected in DC incubated with the same amount of heat-inactivated EIAV. No cytolytic activity was observed after EIAV{sub WSU5} infection of DC. These monocyte-derived DC were more effective than macrophages and B cells in stimulating allogenic T lymphocytes. Both infected macrophages and DC stimulated similar levels of memory CTL responses in mixtures of CD8+ and CD4+ cells as detected with {sup 51}Cr-release assays indicating that EIAV infection of DC did not alter antigen presentation. However, EIAV-infected DC were more effective than infected macrophages when used to stimulate memory CTL in isolated CD8+ cells. The maintenance of antigen processing and presenting function by EIAV-infected DC in vitro suggests that this function is maintained during in vivo infection.

Authors:
 [1];  [2]
  1. Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040 (United States)
  2. Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040 (United States). E-mail: mcguiret@vetmed.wsu.edu
Publication Date:
OSTI Identifier:
20726022
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 335; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2005.02.013; PII: S0042-6822(05)00117-0; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ANEMIAS; ANTIGENS; CHROMIUM 51; IN VITRO; IN VIVO; LYMPHOCYTES; MACROPHAGES; MONOCYTES; VIRUSES

Citation Formats

Rivera, Julie A., and McGuire, Travis C. Equine infectious anemia virus-infected dendritic cells retain antigen presentation capability. United States: N. p., 2005. Web. doi:10.1016/j.virol.2005.02.013.
Rivera, Julie A., & McGuire, Travis C. Equine infectious anemia virus-infected dendritic cells retain antigen presentation capability. United States. doi:10.1016/j.virol.2005.02.013.
Rivera, Julie A., and McGuire, Travis C. Tue . "Equine infectious anemia virus-infected dendritic cells retain antigen presentation capability". United States. doi:10.1016/j.virol.2005.02.013.
@article{osti_20726022,
title = {Equine infectious anemia virus-infected dendritic cells retain antigen presentation capability},
author = {Rivera, Julie A. and McGuire, Travis C.},
abstractNote = {To determine if equine monocyte-derived dendritic cells (DC) were susceptible to equine infectious anemia virus (EIAV) infection, ex vivo-generated DC were infected with virus in vitro. EIAV antigen was detected by immunofluorescence 3 days post-infection with maximum antigen being detected on day 4, whereas there was no antigen detected in DC incubated with the same amount of heat-inactivated EIAV. No cytolytic activity was observed after EIAV{sub WSU5} infection of DC. These monocyte-derived DC were more effective than macrophages and B cells in stimulating allogenic T lymphocytes. Both infected macrophages and DC stimulated similar levels of memory CTL responses in mixtures of CD8+ and CD4+ cells as detected with {sup 51}Cr-release assays indicating that EIAV infection of DC did not alter antigen presentation. However, EIAV-infected DC were more effective than infected macrophages when used to stimulate memory CTL in isolated CD8+ cells. The maintenance of antigen processing and presenting function by EIAV-infected DC in vitro suggests that this function is maintained during in vivo infection.},
doi = {10.1016/j.virol.2005.02.013},
journal = {Virology},
number = 2,
volume = 335,
place = {United States},
year = {Tue May 10 00:00:00 EDT 2005},
month = {Tue May 10 00:00:00 EDT 2005}
}