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Title: Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core

Abstract

Transmissible gastroenteritis coronavirus (TGEV) is one of the most destructive agents, responsible for the enteric infections that are lethal for suckling piglets, causing enormous economic loss to the porcine fostering industry every year. Although it has been known that TGEV spiker protein is essential for the viral entry for many years, the detail knowledge of the TGEV fusion protein core is still very limited. Here, we report that TGEV fusion core (HR1-SGGRGG-HR2), in vitro expressed in GST prokaryotic expression system, shares the typical properties of the trimer of coiled-coil heterodimer (six {alpha}-helix bundle), which has been confirmed by a combined series of biochemical and biophysical evidences including size exclusion chromatography (gel-filtration), chemical crossing, and circular diagram. The 3D homologous structure model presents its most likely structure, extremely similar to those of the coronaviruses documented. Taken together, TGEV spiker protein belongs to the class I fusion protein, characterized by the existence of two heptad-repeat (HR) regions, HR1 and HR2, and the present knowledge about the truncated TGEV fusion protein core may facilitate in the design of the small molecule or polypeptide drugs targeting the membrane fusion between TGEV and its host.

Authors:
 [1];  [2];  [3];  [2];  [3];  [4];  [4];  [5]
  1. Department of Preventive Veterinary, College of Veterinary Medicine, Northeast Agriculture University, 150030 Harbin (China)
  2. Laboratory of Molecular Immunology and Molecular Virology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080 (China)
  3. (China)
  4. China Agricultural University, Beijing 100094 (China)
  5. Department of Preventive Veterinary, College of Veterinary Medicine, Northeast Agriculture University, 150030 Harbin (China). E-mail: yijingli@163.com
Publication Date:
OSTI Identifier:
20713466
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 337; Journal Issue: 4; Other Information: DOI: 10.1016/j.bbrc.2005.09.189; PII: S0006-291X(05)02226-6; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CHROMATOGRAPHY; DRUGS; GELS; IN VITRO; POLYPEPTIDES

Citation Formats

Ma Guangpeng, Feng Youjun, Graduate School of the Chinese Academy of Sciences, Gao Feng, China Agricultural University, Beijing 100094, Wang Jinzi, Liu Cheng, and Li Yijing. Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core. United States: N. p., 2005. Web. doi:10.1016/j.bbrc.2005.09.189.
Ma Guangpeng, Feng Youjun, Graduate School of the Chinese Academy of Sciences, Gao Feng, China Agricultural University, Beijing 100094, Wang Jinzi, Liu Cheng, & Li Yijing. Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core. United States. doi:10.1016/j.bbrc.2005.09.189.
Ma Guangpeng, Feng Youjun, Graduate School of the Chinese Academy of Sciences, Gao Feng, China Agricultural University, Beijing 100094, Wang Jinzi, Liu Cheng, and Li Yijing. Fri . "Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core". United States. doi:10.1016/j.bbrc.2005.09.189.
@article{osti_20713466,
title = {Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core},
author = {Ma Guangpeng and Feng Youjun and Graduate School of the Chinese Academy of Sciences and Gao Feng and China Agricultural University, Beijing 100094 and Wang Jinzi and Liu Cheng and Li Yijing},
abstractNote = {Transmissible gastroenteritis coronavirus (TGEV) is one of the most destructive agents, responsible for the enteric infections that are lethal for suckling piglets, causing enormous economic loss to the porcine fostering industry every year. Although it has been known that TGEV spiker protein is essential for the viral entry for many years, the detail knowledge of the TGEV fusion protein core is still very limited. Here, we report that TGEV fusion core (HR1-SGGRGG-HR2), in vitro expressed in GST prokaryotic expression system, shares the typical properties of the trimer of coiled-coil heterodimer (six {alpha}-helix bundle), which has been confirmed by a combined series of biochemical and biophysical evidences including size exclusion chromatography (gel-filtration), chemical crossing, and circular diagram. The 3D homologous structure model presents its most likely structure, extremely similar to those of the coronaviruses documented. Taken together, TGEV spiker protein belongs to the class I fusion protein, characterized by the existence of two heptad-repeat (HR) regions, HR1 and HR2, and the present knowledge about the truncated TGEV fusion protein core may facilitate in the design of the small molecule or polypeptide drugs targeting the membrane fusion between TGEV and its host.},
doi = {10.1016/j.bbrc.2005.09.189},
journal = {Biochemical and Biophysical Research Communications},
number = 4,
volume = 337,
place = {United States},
year = {Fri Dec 02 00:00:00 EST 2005},
month = {Fri Dec 02 00:00:00 EST 2005}
}