skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Plasma membrane calcium-ATPase 2 and 4 in human breast cancer cell lines

Abstract

There is evidence to suggest that plasma membrane Ca{sup 2+}-ATPase (PMCA) isoforms are important mediators sssof mammary gland physiology. PMCA2 in particular is upregulated extensively during lactation. Expression of other isoforms such as PMCA4 may influence mammary gland epithelial cell proliferation and aberrant regulation of PMCA isoform expression may lead or contribute to mammary gland pathophysiology in the form of breast cancers. To explore whether PMCA2 and PMCA4 expression may be deregulated in breast cancer, we compared mRNA expression of these PMCA isoforms in tumorigenic and non-tumorigenic human breast epithelial cell lines using real time RT-PCR. PMCA2 mRNA has a higher level of expression in some breast cancer cell lines and is overexpressed more than 100-fold in ZR-75-1 cells, compared to non-tumorigenic 184B5 cells. Although differences in PMCA4 mRNA levels were observed between breast cell lines, they were not of the magnitude observed for PMCA2. We conclude that PMCA2 mRNA can be highly overexpressed in some breast cancer cells. The significance of PMCA2 overexpression on tumorigenicity and its possible correlation with other properties such as invasiveness requires further study.

Authors:
 [1];  [1];  [2]
  1. School of Pharmacy, University of Queensland, Brisbane, Qld 4072 (Australia)
  2. School of Pharmacy, University of Queensland, Brisbane, Qld 4072 (Australia). E-mail: G.Monteith@pharmacy.uq.edu.au
Publication Date:
OSTI Identifier:
20713451
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 337; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2005.09.119; PII: S0006-291X(05)02152-2; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CALCIUM; CALCIUM IONS; CARCINOMAS; CELL PROLIFERATION; GENE REGULATION; LACTATION; MAMMARY GLANDS; MEMBRANES; PHYSIOLOGY; POLYMERASE CHAIN REACTION; TRANSCRIPTION

Citation Formats

Lee, Won Jae, Roberts-Thomson, Sarah J., and Monteith, Gregory R. Plasma membrane calcium-ATPase 2 and 4 in human breast cancer cell lines. United States: N. p., 2005. Web. doi:10.1016/j.bbrc.2005.09.119.
Lee, Won Jae, Roberts-Thomson, Sarah J., & Monteith, Gregory R. Plasma membrane calcium-ATPase 2 and 4 in human breast cancer cell lines. United States. doi:10.1016/j.bbrc.2005.09.119.
Lee, Won Jae, Roberts-Thomson, Sarah J., and Monteith, Gregory R. Fri . "Plasma membrane calcium-ATPase 2 and 4 in human breast cancer cell lines". United States. doi:10.1016/j.bbrc.2005.09.119.
@article{osti_20713451,
title = {Plasma membrane calcium-ATPase 2 and 4 in human breast cancer cell lines},
author = {Lee, Won Jae and Roberts-Thomson, Sarah J. and Monteith, Gregory R.},
abstractNote = {There is evidence to suggest that plasma membrane Ca{sup 2+}-ATPase (PMCA) isoforms are important mediators sssof mammary gland physiology. PMCA2 in particular is upregulated extensively during lactation. Expression of other isoforms such as PMCA4 may influence mammary gland epithelial cell proliferation and aberrant regulation of PMCA isoform expression may lead or contribute to mammary gland pathophysiology in the form of breast cancers. To explore whether PMCA2 and PMCA4 expression may be deregulated in breast cancer, we compared mRNA expression of these PMCA isoforms in tumorigenic and non-tumorigenic human breast epithelial cell lines using real time RT-PCR. PMCA2 mRNA has a higher level of expression in some breast cancer cell lines and is overexpressed more than 100-fold in ZR-75-1 cells, compared to non-tumorigenic 184B5 cells. Although differences in PMCA4 mRNA levels were observed between breast cell lines, they were not of the magnitude observed for PMCA2. We conclude that PMCA2 mRNA can be highly overexpressed in some breast cancer cells. The significance of PMCA2 overexpression on tumorigenicity and its possible correlation with other properties such as invasiveness requires further study.},
doi = {10.1016/j.bbrc.2005.09.119},
journal = {Biochemical and Biophysical Research Communications},
number = 3,
volume = 337,
place = {United States},
year = {Fri Nov 25 00:00:00 EST 2005},
month = {Fri Nov 25 00:00:00 EST 2005}
}