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Title: Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic {beta} cells

Abstract

Several nuclear and cytoplasmic proteins in metazoans are modified by O-linked N-acetylglucosamine (O-GlcNAc). This modification is dynamic and reversible similar to phosphorylation and is catalyzed by the O-linked GlcNAc transferase (OGT). Hyperglycemia has been shown to increase O-GlcNAc levels in pancreatic {beta} cells, which appears to interfere with {beta}-cell function. To obtain a better understanding of the role of O-linked GlcNAc modification in {beta} cells, we have isolated OGT interacting proteins from a cDNA library made from the mouse insulinoma MIN6 cell line. We describe here the identification of Ataxin-10, encoded by the SCA10 (spinocerebellar ataxia type 10) gene as an OGT interacting protein. Mutations in the SCA10 gene cause progressive cerebellar ataxias and seizures. We demonstrate that SCA10 interacts with OGT in vivo and is modified by O-linked glycosylation in MIN6 cells, suggesting a novel role for the Ataxin-10 protein in pancreatic {beta} cells.

Authors:
 [1];  [2];  [3]
  1. Department of Molecular and Cellular Biochemistry, University of Kentucky, College of Medicine, 741 South Limestone Street, Lexington, KY 40536 (United States)
  2. Institute for Physiology, University of Basel, 4051 Basel (Switzerland)
  3. Department of Molecular and Cellular Biochemistry, University of Kentucky, College of Medicine, 741 South Limestone Street, Lexington, KY 40536 (United States). E-mail: sozcan@uky.edu
Publication Date:
OSTI Identifier:
20713431
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 337; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.09.026; PII: S0006-291X(05)02031-0; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; GENES; GLUCOSE; HYPERGLYCEMIA; IN VIVO; MICE; MUTATIONS; PANCREAS; PHOSPHORYLATION; PROTEINS

Citation Formats

Andrali, Sreenath S., Maerz, Pia, and Oezcan, Sabire. Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic {beta} cells. United States: N. p., 2005. Web. doi:10.1016/j.bbrc.2005.09.026.
Andrali, Sreenath S., Maerz, Pia, & Oezcan, Sabire. Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic {beta} cells. United States. doi:10.1016/j.bbrc.2005.09.026.
Andrali, Sreenath S., Maerz, Pia, and Oezcan, Sabire. Fri . "Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic {beta} cells". United States. doi:10.1016/j.bbrc.2005.09.026.
@article{osti_20713431,
title = {Ataxin-10 interacts with O-GlcNAc transferase OGT in pancreatic {beta} cells},
author = {Andrali, Sreenath S. and Maerz, Pia and Oezcan, Sabire},
abstractNote = {Several nuclear and cytoplasmic proteins in metazoans are modified by O-linked N-acetylglucosamine (O-GlcNAc). This modification is dynamic and reversible similar to phosphorylation and is catalyzed by the O-linked GlcNAc transferase (OGT). Hyperglycemia has been shown to increase O-GlcNAc levels in pancreatic {beta} cells, which appears to interfere with {beta}-cell function. To obtain a better understanding of the role of O-linked GlcNAc modification in {beta} cells, we have isolated OGT interacting proteins from a cDNA library made from the mouse insulinoma MIN6 cell line. We describe here the identification of Ataxin-10, encoded by the SCA10 (spinocerebellar ataxia type 10) gene as an OGT interacting protein. Mutations in the SCA10 gene cause progressive cerebellar ataxias and seizures. We demonstrate that SCA10 interacts with OGT in vivo and is modified by O-linked glycosylation in MIN6 cells, suggesting a novel role for the Ataxin-10 protein in pancreatic {beta} cells.},
doi = {10.1016/j.bbrc.2005.09.026},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 337,
place = {United States},
year = {Fri Nov 11 00:00:00 EST 2005},
month = {Fri Nov 11 00:00:00 EST 2005}
}