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Title: Nano neodymium oxide induces massive vacuolization and autophagic cell death in non-small cell lung cancer NCI-H460 cells

Abstract

Neodymium, a rare earth element, was known to exhibit cytotoxic effects and induce apoptosis in certain cancer cells. Here we show that nano-sized neodymium oxide (Nano Nd{sub 2}O{sub 3}) induced massive vacuolization and cell death in non-small cell lung cancer NCI-H460 cells at micromolar equivalent concentration range. Cell death elicited by Nano Nd{sub 2}O{sub 3} was not due to apoptosis and caspases were not involved. Electron microscopy and acridine orange staining revealed extensive autophagy in the cytoplasm of the cells treated by Nano Nd{sub 2}O{sub 3}. Autophagy induced by Nano Nd{sub 2}O{sub 3} was accompanied by S-phase cell cycle arrest, mild disruption of mitochondrial membrane potential, and inhibition of proteasome activity. Bafilomycin A1, but not 3-MA, induced apoptosis while inhibiting autophagy. Our results revealed a novel biological function for Nano Nd{sub 2}O{sub 3} and may have implications for the therapy of non-small cell lung cancer.

Authors:
 [1];  [1];  [1];  [2]
  1. Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027 (China)
  2. Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027 (China). E-mail: lpwen@ustc.edu.cn
Publication Date:
OSTI Identifier:
20713427
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 337; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2005.09.018; PII: S0006-291X(05)02002-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; ACRIDINE ORANGE; APOPTOSIS; BIOLOGICAL FUNCTIONS; CARCINOMAS; CELL CYCLE; CYTOPLASM; ELECTRON MICROSCOPY; LUNGS; NEODYMIUM; NEODYMIUM OXIDES; THERAPY

Citation Formats

Chen Yong, Yang Lisong, Feng Chao, and Wen Longping. Nano neodymium oxide induces massive vacuolization and autophagic cell death in non-small cell lung cancer NCI-H460 cells. United States: N. p., 2005. Web. doi:10.1016/j.bbrc.2005.09.018.
Chen Yong, Yang Lisong, Feng Chao, & Wen Longping. Nano neodymium oxide induces massive vacuolization and autophagic cell death in non-small cell lung cancer NCI-H460 cells. United States. doi:10.1016/j.bbrc.2005.09.018.
Chen Yong, Yang Lisong, Feng Chao, and Wen Longping. Fri . "Nano neodymium oxide induces massive vacuolization and autophagic cell death in non-small cell lung cancer NCI-H460 cells". United States. doi:10.1016/j.bbrc.2005.09.018.
@article{osti_20713427,
title = {Nano neodymium oxide induces massive vacuolization and autophagic cell death in non-small cell lung cancer NCI-H460 cells},
author = {Chen Yong and Yang Lisong and Feng Chao and Wen Longping},
abstractNote = {Neodymium, a rare earth element, was known to exhibit cytotoxic effects and induce apoptosis in certain cancer cells. Here we show that nano-sized neodymium oxide (Nano Nd{sub 2}O{sub 3}) induced massive vacuolization and cell death in non-small cell lung cancer NCI-H460 cells at micromolar equivalent concentration range. Cell death elicited by Nano Nd{sub 2}O{sub 3} was not due to apoptosis and caspases were not involved. Electron microscopy and acridine orange staining revealed extensive autophagy in the cytoplasm of the cells treated by Nano Nd{sub 2}O{sub 3}. Autophagy induced by Nano Nd{sub 2}O{sub 3} was accompanied by S-phase cell cycle arrest, mild disruption of mitochondrial membrane potential, and inhibition of proteasome activity. Bafilomycin A1, but not 3-MA, induced apoptosis while inhibiting autophagy. Our results revealed a novel biological function for Nano Nd{sub 2}O{sub 3} and may have implications for the therapy of non-small cell lung cancer.},
doi = {10.1016/j.bbrc.2005.09.018},
journal = {Biochemical and Biophysical Research Communications},
number = 1,
volume = 337,
place = {United States},
year = {Fri Nov 11 00:00:00 EST 2005},
month = {Fri Nov 11 00:00:00 EST 2005}
}