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Title: The suppression of radiation-induced NF-{kappa}B activity by dexamethasone correlates with increased cell death in vivo

Abstract

In this study, we show that dexamethasone treatment increases ionizing radiation-induced cell death by inducing the inhibitory {kappa}B{alpha} (I{kappa}B{alpha}) pathway in mice. The effect of dexamethasone on radiation-induced cell death was assessed by changes in total spleen cellularity and bone marrow colony-forming unit-granulocyte-macrophage (CFU-GM) contents after total body irradiation. While in vivo treatment of mice with dexamethasone alone (1 mg/kg/day, for 2 days) failed to elicit cell death in spleen cells, the combined treatment with dexamethasone (1 mg/kg/day, for 2 days) and {gamma}-rays (1 or 5 Gy) caused a 50-80% reduction in total cellularity in spleen and CFU-GM contents in bone marrow. These results demonstrate that dexamethasone has a synergistic effect on radiation-induced cellular damages in vivo. Immunoblot analysis showed that dexamethasone treatment significantly increases I{kappa}B{alpha} expression in the spleens of irradiated mice. In addition, the dexamethasone treatment significantly reduced radiation-induced nuclear translocation of the nucleus factor-{kappa}B in the spleens of irradiated mice. These results indicate that dexamethasone treatment in vivo may increase radiation-induced cell damages by increasing I{kappa}B{alpha} expression in hematopoietic organs such as spleen and bone marrow.

Authors:
 [1];  [2]
  1. Division of Radiation Effect Research, Radiation Health Research Institute, Korea Hydro and Nuclear Power Co., Seoul 132-703 (Korea, Republic of)
  2. Department of Microbiology, College of Medicine, Hanyang University, Seoul 133-791 (Korea, Republic of). E-mail: hychung@hanyang.ac.kr
Publication Date:
OSTI Identifier:
20713392
Resource Type:
Journal Article
Journal Name:
Biochemical and Biophysical Research Communications
Additional Journal Information:
Journal Volume: 336; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2005.08.135; PII: S0006-291X(05)01768-7; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; APOPTOSIS; BIOLOGICAL RADIATION EFFECTS; BONE MARROW; BONE MARROW CELLS; DEXAMETHASONE; GAMMA RADIATION; IN VIVO; IRRADIATION; LEUKOCYTES; MACROPHAGES; MICE; SPLEEN; SPLEEN CELLS

Citation Formats

Nam, Seon Young, and Chung, Hee-Yong. The suppression of radiation-induced NF-{kappa}B activity by dexamethasone correlates with increased cell death in vivo. United States: N. p., 2005. Web. doi:10.1016/j.bbrc.2005.08.135.
Nam, Seon Young, & Chung, Hee-Yong. The suppression of radiation-induced NF-{kappa}B activity by dexamethasone correlates with increased cell death in vivo. United States. doi:10.1016/j.bbrc.2005.08.135.
Nam, Seon Young, and Chung, Hee-Yong. Fri . "The suppression of radiation-induced NF-{kappa}B activity by dexamethasone correlates with increased cell death in vivo". United States. doi:10.1016/j.bbrc.2005.08.135.
@article{osti_20713392,
title = {The suppression of radiation-induced NF-{kappa}B activity by dexamethasone correlates with increased cell death in vivo},
author = {Nam, Seon Young and Chung, Hee-Yong},
abstractNote = {In this study, we show that dexamethasone treatment increases ionizing radiation-induced cell death by inducing the inhibitory {kappa}B{alpha} (I{kappa}B{alpha}) pathway in mice. The effect of dexamethasone on radiation-induced cell death was assessed by changes in total spleen cellularity and bone marrow colony-forming unit-granulocyte-macrophage (CFU-GM) contents after total body irradiation. While in vivo treatment of mice with dexamethasone alone (1 mg/kg/day, for 2 days) failed to elicit cell death in spleen cells, the combined treatment with dexamethasone (1 mg/kg/day, for 2 days) and {gamma}-rays (1 or 5 Gy) caused a 50-80% reduction in total cellularity in spleen and CFU-GM contents in bone marrow. These results demonstrate that dexamethasone has a synergistic effect on radiation-induced cellular damages in vivo. Immunoblot analysis showed that dexamethasone treatment significantly increases I{kappa}B{alpha} expression in the spleens of irradiated mice. In addition, the dexamethasone treatment significantly reduced radiation-induced nuclear translocation of the nucleus factor-{kappa}B in the spleens of irradiated mice. These results indicate that dexamethasone treatment in vivo may increase radiation-induced cell damages by increasing I{kappa}B{alpha} expression in hematopoietic organs such as spleen and bone marrow.},
doi = {10.1016/j.bbrc.2005.08.135},
journal = {Biochemical and Biophysical Research Communications},
issn = {0006-291X},
number = 2,
volume = 336,
place = {United States},
year = {2005},
month = {10}
}