The mouse CCR2 gene is regulated by two promoters that are responsive to plasma cholesterol and peroxisome proliferator-activated receptor {gamma} ligands

Journal Article · Fri Jun 24 00:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.04.110· OSTI ID:20710821

Yiming, Chen ^[1]; Green, Simone R ^[1]; Ho, Jessica ^[1]; Li, Andrew ^[1]; Almazan, Felizidad ^[1]; Quehenberger, Oswald ^[1]

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1. Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0682 (United States)

We have previously shown that the expression of monocyte CCR2, the receptor for monocyte chemoattractant protein-1, is induced by plasma cholesterol. The present study examines the mechanisms that regulate monocyte CCR2 expression in hypercholesterolemia using a mouse model. Our data demonstrate that in the mouse, CCR2 expression in circulating monocytes is controlled by two promoters P1 and P2. The two distinct transcripts, which encode the same protein, are produced by alternative splicing in the 5'-untranslated region. Both promoters are constitutively active, but only P2 is stimulated by cholesterol. However, both promoters are repressed by peroxisome proliferator-activated receptor {gamma}.

• OSTI ID: 20710821
• Journal Information: Biochemical and Biophysical Research Communications, Vol. 332, Issue 1; Other Information: DOI: 10.1016/j.bbrc.2005.04.110; PII: S0006-291X(05)00893-4; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ARTERIOSCLEROSIS
CHOLESTEROL
GENES
LIGANDS
MICE
MONOCYTES
RECEPTORS
SPLICING