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Title: Apoptosis and proliferation of oligodendrocyte progenitor cells in the irradiated rodent spinal cord

Abstract

Purpose: Oligodendrocytes undergo early apoptosis after irradiation. The aim of this study was to determine the relationship between oligodendroglial apoptosis and proliferation of oligodendrocyte progenitor cells (OPC) in the irradiated central nervous system. Methods and Materials: Adult rats and p53 transgenic mice were given single doses of 2 Gy, 8 Gy, or 22 Gy to the cervical spinal cord. Apoptosis was assessed using TUNEL (Tdt-mediated dUTP terminal nick-end labeling) staining or by examining nuclear morphology. Oligodendrocyte progenitor cells were identified with an NG2 antibody or by in situ hybridization for platelet-derived growth factor receptor {alpha}. Proliferation of OPC was assessed by in vivo bromodeoxyuridine (BrdU) labeling and subsequent immunohistochemistry. Because radiation-induced apoptosis of oligodendroglial cells is p53 dependent, p53 transgenic mice were used to study the relationship between apoptosis and cell proliferation. Results: Oligodendrocyte progenitor cells underwent apoptosis within 24 h of irradiation in the rat. That did not result in a change in OPC density at 24 h. Oligodendrocyte progenitor cell density was significantly reduced by 2-4 weeks, but showed recovery by 6 weeks after irradiation. An increase in BrdU-labeled cells was observed at 2 weeks after 8 Gy or 22 Gy, and proliferating cells in the rat spinalmore » cord were immunoreactive for NG2. The mouse spinal cord showed a similar early cell proliferation after irradiation. No difference was observed in the proliferation response in the spinal cord of p53 -/- mice compared with wild type animals. Conclusions: Oligodendroglial cells undergo early apoptosis and OPC undergo early proliferation after ionizing radiation. However, apoptosis is not likely to be the trigger for early proliferation of OPC in the irradiated central nervous system.« less

Authors:
 [1];  [1];  [2]
  1. Discipline of Molecular and Cell Biology, Sunnybrook and Women's College Health Sciences Center, University of Toronto, Toronto, ON (Canada)
  2. Department of Radiation Oncology, Sunnybrook and Women's College Health Sciences Center, University of Toronto, Toronto, ON (Canada). E-mail: shun.wong@sw.ca
Publication Date:
OSTI Identifier:
20698470
Resource Type:
Journal Article
Resource Relation:
Journal Name: International Journal of Radiation Oncology, Biology and Physics; Journal Volume: 62; Journal Issue: 2; Other Information: DOI: 10.1016/j.ijrobp.2005.01.061; PII: S0360-3016(05)00235-X; Copyright (c) 2005 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANTIBODIES; APOPTOSIS; CELL PROLIFERATION; GROWTH FACTORS; IN VIVO; IN-SITU HYBRIDIZATION; IONIZING RADIATIONS; IRRADIATION; MORPHOLOGY; RADIATION INJURIES; RATS; RECEPTORS; SPINAL CORD; TRANSGENIC MICE

Citation Formats

Atkinson, Shelley L., Li Yuqing, and Wong, C. Shun. Apoptosis and proliferation of oligodendrocyte progenitor cells in the irradiated rodent spinal cord. United States: N. p., 2005. Web. doi:10.1016/j.ijrobp.2005.01.061.
Atkinson, Shelley L., Li Yuqing, & Wong, C. Shun. Apoptosis and proliferation of oligodendrocyte progenitor cells in the irradiated rodent spinal cord. United States. doi:10.1016/j.ijrobp.2005.01.061.
Atkinson, Shelley L., Li Yuqing, and Wong, C. Shun. Wed . "Apoptosis and proliferation of oligodendrocyte progenitor cells in the irradiated rodent spinal cord". United States. doi:10.1016/j.ijrobp.2005.01.061.
@article{osti_20698470,
title = {Apoptosis and proliferation of oligodendrocyte progenitor cells in the irradiated rodent spinal cord},
author = {Atkinson, Shelley L. and Li Yuqing and Wong, C. Shun},
abstractNote = {Purpose: Oligodendrocytes undergo early apoptosis after irradiation. The aim of this study was to determine the relationship between oligodendroglial apoptosis and proliferation of oligodendrocyte progenitor cells (OPC) in the irradiated central nervous system. Methods and Materials: Adult rats and p53 transgenic mice were given single doses of 2 Gy, 8 Gy, or 22 Gy to the cervical spinal cord. Apoptosis was assessed using TUNEL (Tdt-mediated dUTP terminal nick-end labeling) staining or by examining nuclear morphology. Oligodendrocyte progenitor cells were identified with an NG2 antibody or by in situ hybridization for platelet-derived growth factor receptor {alpha}. Proliferation of OPC was assessed by in vivo bromodeoxyuridine (BrdU) labeling and subsequent immunohistochemistry. Because radiation-induced apoptosis of oligodendroglial cells is p53 dependent, p53 transgenic mice were used to study the relationship between apoptosis and cell proliferation. Results: Oligodendrocyte progenitor cells underwent apoptosis within 24 h of irradiation in the rat. That did not result in a change in OPC density at 24 h. Oligodendrocyte progenitor cell density was significantly reduced by 2-4 weeks, but showed recovery by 6 weeks after irradiation. An increase in BrdU-labeled cells was observed at 2 weeks after 8 Gy or 22 Gy, and proliferating cells in the rat spinal cord were immunoreactive for NG2. The mouse spinal cord showed a similar early cell proliferation after irradiation. No difference was observed in the proliferation response in the spinal cord of p53 -/- mice compared with wild type animals. Conclusions: Oligodendroglial cells undergo early apoptosis and OPC undergo early proliferation after ionizing radiation. However, apoptosis is not likely to be the trigger for early proliferation of OPC in the irradiated central nervous system.},
doi = {10.1016/j.ijrobp.2005.01.061},
journal = {International Journal of Radiation Oncology, Biology and Physics},
number = 2,
volume = 62,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 2005},
month = {Wed Jun 01 00:00:00 EDT 2005}
}