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Title: Induction of endothelial cell proliferation by angiogenic factors released by activated monocytes

Abstract

Introduction: Cell-cell interaction is an essential component of atherosclerotic plaque development. Activated monocytes appear to play a central role in the development of atherosclerosis, not only through foam cell formation but also via the production of various growth factors that induce proliferation of different cell types that are involved in the plaque development. Using serum free co-culture method, we determined the effect of monocytes on endothelial cell proliferation. Methods: Endothelial cell proliferation is determined by the amount of [{sup 3}H]thymidine incorporated in to the DNA. Basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) levels in the conditioned medium were determined by ELISA. Results: Conditioned medium from unactivated monocytes partially inhibited endothelial cell proliferation, whereas conditioned medium from activated monocytes promoted endothelial cell proliferation. The mitogenic effect of conditioned medium derived from activated monocytes is due to the presence of b-FGF, VEGF and IL-8. Neutralizing antibodies against b-FGF, VEGF and IL-8 partially reversed the mitogenic effect of conditioned medium derived from activated monocytes. When b-FGF, VEGF and IL-8 were immunoprecipitated from conditioned medium derived from activated monocytes, it is less mitogenic to endothelial cells. Conclusion: Activated monocytes may play an important role in the development ofmore » atherosclerotic plaque by producing endothelial cell growth factors.« less

Authors:
; ;
Publication Date:
OSTI Identifier:
20650547
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cardiovascular Radiation Medicine; Journal Volume: 3; Journal Issue: 2; Other Information: DOI: 10.1016/S1522-1865(02)00159-2; PII: S1522186502001592; Copyright (c) 2003 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIBODIES; ARTERIOSCLEROSIS; CELL PROLIFERATION; DNA; ENZYME IMMUNOASSAY; FIBROBLASTS; GROWTH FACTORS; MONOCYTES; THYMIDINE; TRITIUM

Citation Formats

Pakala, Rajbabu, Watanabe, Takuya, and Benedict, Claude R. Induction of endothelial cell proliferation by angiogenic factors released by activated monocytes. United States: N. p., 2002. Web. doi:10.1016/S1522-1865(02)00159-2.
Pakala, Rajbabu, Watanabe, Takuya, & Benedict, Claude R. Induction of endothelial cell proliferation by angiogenic factors released by activated monocytes. United States. doi:10.1016/S1522-1865(02)00159-2.
Pakala, Rajbabu, Watanabe, Takuya, and Benedict, Claude R. Sat . "Induction of endothelial cell proliferation by angiogenic factors released by activated monocytes". United States. doi:10.1016/S1522-1865(02)00159-2.
@article{osti_20650547,
title = {Induction of endothelial cell proliferation by angiogenic factors released by activated monocytes},
author = {Pakala, Rajbabu and Watanabe, Takuya and Benedict, Claude R},
abstractNote = {Introduction: Cell-cell interaction is an essential component of atherosclerotic plaque development. Activated monocytes appear to play a central role in the development of atherosclerosis, not only through foam cell formation but also via the production of various growth factors that induce proliferation of different cell types that are involved in the plaque development. Using serum free co-culture method, we determined the effect of monocytes on endothelial cell proliferation. Methods: Endothelial cell proliferation is determined by the amount of [{sup 3}H]thymidine incorporated in to the DNA. Basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) levels in the conditioned medium were determined by ELISA. Results: Conditioned medium from unactivated monocytes partially inhibited endothelial cell proliferation, whereas conditioned medium from activated monocytes promoted endothelial cell proliferation. The mitogenic effect of conditioned medium derived from activated monocytes is due to the presence of b-FGF, VEGF and IL-8. Neutralizing antibodies against b-FGF, VEGF and IL-8 partially reversed the mitogenic effect of conditioned medium derived from activated monocytes. When b-FGF, VEGF and IL-8 were immunoprecipitated from conditioned medium derived from activated monocytes, it is less mitogenic to endothelial cells. Conclusion: Activated monocytes may play an important role in the development of atherosclerotic plaque by producing endothelial cell growth factors.},
doi = {10.1016/S1522-1865(02)00159-2},
journal = {Cardiovascular Radiation Medicine},
number = 2,
volume = 3,
place = {United States},
year = {Sat Jun 01 00:00:00 EDT 2002},
month = {Sat Jun 01 00:00:00 EDT 2002}
}