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Title: Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B

Abstract

Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-{kappa}B). The activation of NF-{kappa}B has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a transcriptional processivity and anti-termination factor, is sufficient to activate NF-{kappa}B in A549 cells. Electromobility shift assays show increased NF-{kappa}B complexes in the nuclei of M2-1-expressing cells. M2-1 protein is found in nuclei of M2-1-expressing cells and in RSV-infected cells. Co-immunoprecipitations of nuclear extracts of M2-1-expressing cells and of RSV-infected cells revealed an association of M2-1 with Rel A protein. Furthermore, the activation of NF-{kappa}B depends on the C-terminus of the RSV M2-1 protein, as shown by NF-{kappa}B-induced gene expression of a reporter gene construct.

Authors:
 [1];  [2];  [2]
  1. Klinik fuer Plastische, Hand-und Wiederherstellungschirurgie, Podbielskistrasse 380, D-30659 Hannover (Germany). E-mail: reimers.kerstin@mh-hannover.de
  2. Institut fuer Medizinische Mikrobiologie, Otto-von-Guericke-Universitaet Magdeburg, Leipzigerstrasse 44, D-39120 Magdeburg (Germany)
Publication Date:
OSTI Identifier:
20637195
Resource Type:
Journal Article
Resource Relation:
Journal Name: Virology; Journal Volume: 331; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2004.10.031; PII: S0042-6822(04)00682-8; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL NUCLEI; GENES; INFECTIOUS DISEASES; LYMPHOKINES; RESPIRATORY SYSTEM DISEASES; VIRUSES

Citation Formats

Reimers, Kerstin, Buchholz, Katja, and Werchau, Hermann. Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B. United States: N. p., 2005. Web. doi:10.1016/j.virol.2004.10.031.
Reimers, Kerstin, Buchholz, Katja, & Werchau, Hermann. Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B. United States. doi:10.1016/j.virol.2004.10.031.
Reimers, Kerstin, Buchholz, Katja, and Werchau, Hermann. Thu . "Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B". United States. doi:10.1016/j.virol.2004.10.031.
@article{osti_20637195,
title = {Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B},
author = {Reimers, Kerstin and Buchholz, Katja and Werchau, Hermann},
abstractNote = {Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-{kappa}B). The activation of NF-{kappa}B has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a transcriptional processivity and anti-termination factor, is sufficient to activate NF-{kappa}B in A549 cells. Electromobility shift assays show increased NF-{kappa}B complexes in the nuclei of M2-1-expressing cells. M2-1 protein is found in nuclei of M2-1-expressing cells and in RSV-infected cells. Co-immunoprecipitations of nuclear extracts of M2-1-expressing cells and of RSV-infected cells revealed an association of M2-1 with Rel A protein. Furthermore, the activation of NF-{kappa}B depends on the C-terminus of the RSV M2-1 protein, as shown by NF-{kappa}B-induced gene expression of a reporter gene construct.},
doi = {10.1016/j.virol.2004.10.031},
journal = {Virology},
number = 2,
volume = 331,
place = {United States},
year = {Thu Jan 20 00:00:00 EST 2005},
month = {Thu Jan 20 00:00:00 EST 2005}
}