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Title: HPV-18 confers resistance to TNF-{alpha} in organotypic cultures of human keratinocytes

Abstract

The proinflammatory cytokine tumor necrosis factor-alpha (TNF-{alpha}) inhibits normal keratinocytes proliferation. However, many human papillomavirus (HPV)-immortalized or transformed cell lines are resistant to TNF-{alpha} antiproliferative effect. The present study analyzes the effects of TNF-{alpha} on organotypic cultures of primary human keratinocytes (PHKs) that express HPV-18 oncogenes. Raft cultures prepared with PHKs acutely transfected with HPV-18 whole genome or infected with recombinant retroviruses containing only E6/E7 or E7 were treated with 2 nM TNF-{alpha}. While BrdU incorporation into basal/parabasal cells of normal PHKs cultures was markedly inhibited by TNF-{alpha} cultures transfected with HPV-18 whole genome showed proliferation in all cell strata. Furthermore, BrdU incorporation into cultures expressing E6/E7 or E7 was not significantly reduced, indicating that E7 alone confers partial resistance to TNF-{alpha}. Besides, TNF-{alpha} treatment did not alter p16{sup ink4a}, p21{sup cip1}, p27{sup kip1}, or cyclin E levels, but did reduce cyclin A and PCNA levels in sensitive cells.

Authors:
 [1];  [2];  [2];  [2];  [3]
  1. Ludwig Institute for Cancer Research, 1509-010 Sao Paulo, SP (Brazil) and Instituto de Quimica, Universidade de Sao Paulo, 05508-900 Sao Paulo, SP (Brazil)
  2. Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL 35294-0005 (United States)
  3. Ludwig Institute for Cancer Research, 1509-010 Sao Paulo, SP (Brazil)
Publication Date:
OSTI Identifier:
20634886
Resource Type:
Journal Article
Journal Name:
Virology
Additional Journal Information:
Journal Volume: 328; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2004.07.026; PII: S0042-6822(04)00496-9; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0042-6822
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; CELL NUCLEI; CELL PROLIFERATION; HAZARDS; ONCOGENES; VIRUSES

Citation Formats

Boccardo, Enrique, Noya, Francisco, Broker, Thomas R, Chow, Louise T, and Villa, Luisa L. HPV-18 confers resistance to TNF-{alpha} in organotypic cultures of human keratinocytes. United States: N. p., 2004. Web. doi:10.1016/j.virol.2004.07.026.
Boccardo, Enrique, Noya, Francisco, Broker, Thomas R, Chow, Louise T, & Villa, Luisa L. HPV-18 confers resistance to TNF-{alpha} in organotypic cultures of human keratinocytes. United States. https://doi.org/10.1016/j.virol.2004.07.026
Boccardo, Enrique, Noya, Francisco, Broker, Thomas R, Chow, Louise T, and Villa, Luisa L. 2004. "HPV-18 confers resistance to TNF-{alpha} in organotypic cultures of human keratinocytes". United States. https://doi.org/10.1016/j.virol.2004.07.026.
@article{osti_20634886,
title = {HPV-18 confers resistance to TNF-{alpha} in organotypic cultures of human keratinocytes},
author = {Boccardo, Enrique and Noya, Francisco and Broker, Thomas R and Chow, Louise T and Villa, Luisa L},
abstractNote = {The proinflammatory cytokine tumor necrosis factor-alpha (TNF-{alpha}) inhibits normal keratinocytes proliferation. However, many human papillomavirus (HPV)-immortalized or transformed cell lines are resistant to TNF-{alpha} antiproliferative effect. The present study analyzes the effects of TNF-{alpha} on organotypic cultures of primary human keratinocytes (PHKs) that express HPV-18 oncogenes. Raft cultures prepared with PHKs acutely transfected with HPV-18 whole genome or infected with recombinant retroviruses containing only E6/E7 or E7 were treated with 2 nM TNF-{alpha}. While BrdU incorporation into basal/parabasal cells of normal PHKs cultures was markedly inhibited by TNF-{alpha} cultures transfected with HPV-18 whole genome showed proliferation in all cell strata. Furthermore, BrdU incorporation into cultures expressing E6/E7 or E7 was not significantly reduced, indicating that E7 alone confers partial resistance to TNF-{alpha}. Besides, TNF-{alpha} treatment did not alter p16{sup ink4a}, p21{sup cip1}, p27{sup kip1}, or cyclin E levels, but did reduce cyclin A and PCNA levels in sensitive cells.},
doi = {10.1016/j.virol.2004.07.026},
url = {https://www.osti.gov/biblio/20634886}, journal = {Virology},
issn = {0042-6822},
number = 2,
volume = 328,
place = {United States},
year = {Mon Oct 25 00:00:00 EDT 2004},
month = {Mon Oct 25 00:00:00 EDT 2004}
}