Magnetic fields at resonant conditions for the hydrogen ion affect neurite outgrowth in PC-12 cells: A test of the ion parametric resonance model
- Hospital Ramon y Cajal, Madrid (Spain). Dept. Investigacion
- Bechtel Corp., San Francisco, CA (United States). Research and Development Dept.
- Environmental Protection Agency, Research Triangle Park, NC (United States)
PC-12 cells primed with nerve growth factor (NGF) were exposed to sinusoidal extremely-low-frequency (ELF) magnetic fields (MFs) selected to test the predictions of the ion parametric resonance (IPR) model under resonance conditions for a single ion (hydrogen). The authors examined the field effects on the neurite outgrowth (NO) induced by NGF using three different combinations of flux densities of the parallel components of the AC MF (B{sub ac}) and the static MF (B{sub dc}). The first test examined the NO response in cells exposed to 45 Hz at a B{sub dc} of 2.96 {micro}T with resonant conditions for H{sup +} according to the model. The B{sub ac} values ranged from 0.29 to 4.11 {micro}T root-mean-square (rms). In the second test, the MF effects at off-resonance conditions (i.e., no biologically significant ion at resonance) were examined using the frequency of 45 Hz with a B{sub dc} of 1.97 {micro}T and covering a B{sub ac} range between 0.79 and 2.05 {micro}T rms. In the third test, the Ac frequency was changed to 30 Hz with the subsequent change in B{sub dc} to 1.97 {micro}T to tune for H{sup +} as in the first test. The B{sub ac} values ranged from 0.79 to 2.05 {micro}T rms. After a 23 h incubation and exposure to the MF in the presence of NGF (5 ng/ml), the NO was analyzed using a stereoscopic microscope. The results showed that the NGF stimulation of neurite outgrowth (NSNO) was affected by MF combinations over most of the B{sub ac} exposure range generally consistent with the predictions of the IPR model. However, for a distinct range of B{sub ac} where the IPR model predicted maximal ionic influence, the observed pattern of NSNO contrasted sharply with those predictions. The symmetry of this response suggests that values of B{sub ac} within this distinct range may trigger alternate or additional cellular mechanisms that lead to an apparent lack of response to the MF stimulus.
- DOE Contract Number:
- AI01-89CE34024
- OSTI ID:
- 205268
- Journal Information:
- Bioelectromagnetics, Vol. 17, Issue 1; Other Information: PBD: 1996
- Country of Publication:
- United States
- Language:
- English
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