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Title: Fluorescence imaging of glutamate release in neurons

Abstract

A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with charge-coupled device (CCD) imaging is down to {mu}M levels of glutamate with reasonable response time ({approx}30 s). The standard glutamate test shows a linear response over 3 orders of magnitude, from {mu}M to 0.1 mM range. The in vitro monitoring of glutamate release from cultured neuron cells demonstrated excellent spatial and temporal resolution. (c) 1999 Society for Applied Spectroscopy.

Authors:
 [1];  [1]
  1. Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011 (United States)
Publication Date:
OSTI Identifier:
20216187
Resource Type:
Journal Article
Journal Name:
Applied Spectroscopy
Additional Journal Information:
Journal Volume: 53; Journal Issue: 12; Other Information: PBD: Dec 1999; Journal ID: ISSN 0003-7028
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; FLUORESCENCE SPECTROSCOPY; NERVE CELLS; CHARGE-COUPLED DEVICES; CALIBRATION; GLUTAMINE; CYTOCHEMISTRY; EXPERIMENTAL DATA

Citation Formats

Wang, Ziqiang, and Yeung, Edward S. Fluorescence imaging of glutamate release in neurons. United States: N. p., 1999. Web. doi:10.1366/0003702991946235.
Wang, Ziqiang, & Yeung, Edward S. Fluorescence imaging of glutamate release in neurons. United States. doi:10.1366/0003702991946235.
Wang, Ziqiang, and Yeung, Edward S. Wed . "Fluorescence imaging of glutamate release in neurons". United States. doi:10.1366/0003702991946235.
@article{osti_20216187,
title = {Fluorescence imaging of glutamate release in neurons},
author = {Wang, Ziqiang and Yeung, Edward S},
abstractNote = {A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with charge-coupled device (CCD) imaging is down to {mu}M levels of glutamate with reasonable response time ({approx}30 s). The standard glutamate test shows a linear response over 3 orders of magnitude, from {mu}M to 0.1 mM range. The in vitro monitoring of glutamate release from cultured neuron cells demonstrated excellent spatial and temporal resolution. (c) 1999 Society for Applied Spectroscopy.},
doi = {10.1366/0003702991946235},
journal = {Applied Spectroscopy},
issn = {0003-7028},
number = 12,
volume = 53,
place = {United States},
year = {1999},
month = {12}
}