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Title: Carbon tetrachloride degradation: Effect of microbial growth substrate and vitamin B{sub 12} content

Abstract

Microbial degradation kinetics of carbon tetrachloride (CT) under reducing conditions were investigated for different cultures, fed with 1,2-propanediol, dextrose, propionalde-hyde, or acetate and nitrate, in the anaerobic step of an anaerobic/aerobic operation sequence. Methanogenesis was inhibited due to the aerobic step. CT biodegradation rates followed first-order kinetics with respect to CT concentration and biomass and were not affected by the presence of growth substrate. CT degradation rates increased linearly with higher intracellular vitamin B{sub 12} content. The culture fed 1,2-propanediol had the highest vitamin B{sub 12} content, which was 3.8, 4.7, and 16 times that of the propionaldehyde-,dextrose-, and acetate-fed cultures, respectively, and its first-order degradation rate constant was 2.8, 4.5, 6.0 times that for those cultures, respectively. No CT degradation occurred with culture liquid, suggesting that intracellular factors were responsible for CT degradation. The propanediol culture was able to sustain a constant CT degradation rate for a 16-day test period without substrate addition. Compared to a propanediol-fed culture grown only under anaerobic conditions, the propanediol culture grown under the sequential anaerobic/aerobic condition resulted in more biomass growth and a greater CT degradation rate per unit of propanediol fed, although its CT degradation rate per unit of biomass was lower.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Washington, Seattle, WA (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
20080516
DOE Contract Number:  
FC01-95EW55084
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Science and Technology; Journal Volume: 34; Journal Issue: 9; Other Information: PBD: 1 May 2000
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; REMEDIAL ACTION; CARBON TETRACHLORIDE; GROWTH; VITAMIN B-12; AEROBIC CONDITIONS; ANAEROBIC CONDITIONS

Citation Formats

Zou, S., Stensel, H.D., and Ferguson, J.F. Carbon tetrachloride degradation: Effect of microbial growth substrate and vitamin B{sub 12} content. United States: N. p., 2000. Web. doi:10.1021/es990930m.
Zou, S., Stensel, H.D., & Ferguson, J.F. Carbon tetrachloride degradation: Effect of microbial growth substrate and vitamin B{sub 12} content. United States. doi:10.1021/es990930m.
Zou, S., Stensel, H.D., and Ferguson, J.F. Mon . "Carbon tetrachloride degradation: Effect of microbial growth substrate and vitamin B{sub 12} content". United States. doi:10.1021/es990930m.
@article{osti_20080516,
title = {Carbon tetrachloride degradation: Effect of microbial growth substrate and vitamin B{sub 12} content},
author = {Zou, S. and Stensel, H.D. and Ferguson, J.F.},
abstractNote = {Microbial degradation kinetics of carbon tetrachloride (CT) under reducing conditions were investigated for different cultures, fed with 1,2-propanediol, dextrose, propionalde-hyde, or acetate and nitrate, in the anaerobic step of an anaerobic/aerobic operation sequence. Methanogenesis was inhibited due to the aerobic step. CT biodegradation rates followed first-order kinetics with respect to CT concentration and biomass and were not affected by the presence of growth substrate. CT degradation rates increased linearly with higher intracellular vitamin B{sub 12} content. The culture fed 1,2-propanediol had the highest vitamin B{sub 12} content, which was 3.8, 4.7, and 16 times that of the propionaldehyde-,dextrose-, and acetate-fed cultures, respectively, and its first-order degradation rate constant was 2.8, 4.5, 6.0 times that for those cultures, respectively. No CT degradation occurred with culture liquid, suggesting that intracellular factors were responsible for CT degradation. The propanediol culture was able to sustain a constant CT degradation rate for a 16-day test period without substrate addition. Compared to a propanediol-fed culture grown only under anaerobic conditions, the propanediol culture grown under the sequential anaerobic/aerobic condition resulted in more biomass growth and a greater CT degradation rate per unit of propanediol fed, although its CT degradation rate per unit of biomass was lower.},
doi = {10.1021/es990930m},
journal = {Environmental Science and Technology},
number = 9,
volume = 34,
place = {United States},
year = {Mon May 01 00:00:00 EDT 2000},
month = {Mon May 01 00:00:00 EDT 2000}
}