Effects of acute exposure to fluoranthene-contaminated sediment on the survival and genetic variability of fathead minnows (Pimephales promelas)
One-month-old fathead minnows (Pimephales promelas) were exposed to fluoranthene-contaminated sediment during a 96-h exposure. Of the 909 minnows exposed to fluoranthene, 684 (75%) minnows died during the exposure. Horizontal starch-gel electrophoresis was used to determine genotypes at six variable enzyme loci ({beta}-GAL*, GPI-1*, GPI-2*, IDHP-1*, MDH-2*, and PGM*). Statistical analyses were used to evaluate the relationships between the genetic data and weight, length, and time to death (TTD) of fish using an accelerated failure time regression model (LIFEREG). The GPI-1*, MDH-2*, and PGM* loci were found to be significantly related to TTD. Multilocus heterozygosity was also related to TTD. Lower heterozygosity was related to a longer TTD and a greater chance of survival. Mean fish weight differed significantly among genotypes at each locus. This resulted in large differences between LIFEREG regression models that factored weight out and those models that die not separate weight from the genotypes or multilocus heterozygosity. The results of the study indicated that differential survival to fluoranthene was genetically related. The frequencies of several genotypes were significantly different in the survivors of the fluoranthene exposure compared with those in the initial population.
- Research Organization:
- Miami Univ., Oxford, OH (US)
- OSTI ID:
- 20080487
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 19, Issue 4; Other Information: PBD: Apr 2000; ISSN 0730-7268
- Country of Publication:
- United States
- Language:
- English
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