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Title: Determination of polycyclic aromatic hydrocarbons in contaminated water and soil samples by immunological and chromatographic methods

Abstract

An immunoassay was developed that can be used for the detection of polycyclic aromatic hydrocarbons (PAHs) in water, landfill leachate, and soil. As test format an indirect competitive microtiter plate ELISA (enzyme-linked immunosorbent assay) was applied. While groundwater samples from a former manufactured gas plant site could be analyzed directly, soil and landfill leachate had to be extracted and required at least a 100-fold dilution prior to immunochemical measurement. PAHs could be recovered from fortified reference soils as well as aged field samples with high yield using 1-h ultrasonication with acetonitrile. Extraction efficiency was comparable to Soxhlet extraction and ultrasonication with tetrahydrogurane. Recovery was lower with agitation but would still be acceptable for use in an on-site field test to provide rapid, semiquantitative, and reliable test results for making environmental decisions such as identifying hot spots, site mapping, monitoring of remediation processes, and selecting site samples for laboratory analysis. Classification of ELISA data showed that it was possible to estimate the PAH contamination in soils with about 5% false positive and 5% false negative results that may have arisen from heterogeneity of samples, cross-reactivity of compounds with a similar structure, humic acids, or unknown interferences.

Authors:
; ; ;
Publication Date:
Research Org.:
Technical Univ. Munich (DE)
OSTI Identifier:
20080466
Resource Type:
Journal Article
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 34; Journal Issue: 10; Other Information: PBD: 15 May 2000; Journal ID: ISSN 0013-936X
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; SOILS; GROUND WATER; POLYCYCLIC AROMATIC HYDROCARBONS; ENZYME IMMUNOASSAY; REMEDIAL ACTION; SITE CHARACTERIZATION; MONITORING; CHROMATOGRAPHY

Citation Formats

Knopp, D., Seifert, M., Vaeaenaenen, V., and Niessner, R. Determination of polycyclic aromatic hydrocarbons in contaminated water and soil samples by immunological and chromatographic methods. United States: N. p., 2000. Web. doi:10.1021/es991215f.
Knopp, D., Seifert, M., Vaeaenaenen, V., & Niessner, R. Determination of polycyclic aromatic hydrocarbons in contaminated water and soil samples by immunological and chromatographic methods. United States. doi:10.1021/es991215f.
Knopp, D., Seifert, M., Vaeaenaenen, V., and Niessner, R. Mon . "Determination of polycyclic aromatic hydrocarbons in contaminated water and soil samples by immunological and chromatographic methods". United States. doi:10.1021/es991215f.
@article{osti_20080466,
title = {Determination of polycyclic aromatic hydrocarbons in contaminated water and soil samples by immunological and chromatographic methods},
author = {Knopp, D. and Seifert, M. and Vaeaenaenen, V. and Niessner, R.},
abstractNote = {An immunoassay was developed that can be used for the detection of polycyclic aromatic hydrocarbons (PAHs) in water, landfill leachate, and soil. As test format an indirect competitive microtiter plate ELISA (enzyme-linked immunosorbent assay) was applied. While groundwater samples from a former manufactured gas plant site could be analyzed directly, soil and landfill leachate had to be extracted and required at least a 100-fold dilution prior to immunochemical measurement. PAHs could be recovered from fortified reference soils as well as aged field samples with high yield using 1-h ultrasonication with acetonitrile. Extraction efficiency was comparable to Soxhlet extraction and ultrasonication with tetrahydrogurane. Recovery was lower with agitation but would still be acceptable for use in an on-site field test to provide rapid, semiquantitative, and reliable test results for making environmental decisions such as identifying hot spots, site mapping, monitoring of remediation processes, and selecting site samples for laboratory analysis. Classification of ELISA data showed that it was possible to estimate the PAH contamination in soils with about 5% false positive and 5% false negative results that may have arisen from heterogeneity of samples, cross-reactivity of compounds with a similar structure, humic acids, or unknown interferences.},
doi = {10.1021/es991215f},
journal = {Environmental Science and Technology},
issn = {0013-936X},
number = 10,
volume = 34,
place = {United States},
year = {2000},
month = {5}
}