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Title: Application of capillary electrophoresis to anion speciation in soil water extracts: 2. Arsenic

Abstract

A method has been developed for the speciation of arsenic (AsO{sub 2}{sup {minus}}, AsO{sub 4}{sup 3{minus}}, and dimethylarsinic [DMA]) in natural soil solutions from contaminated sites in Australia. The separation of these anions was achieved by capillary zone electrophoresis (CZE) using a fused silica capillary with a basic chromate buffer and on-column indirect UV detection at 254 nm. Method parameters, such as electrolyte pH, run voltage, and capillary temperature were studied in order to establish suitable analytical conditions. The ideal separation for As(III) and DMA was achieved with a buffer pH of 8.0, a run voltage of 25 kV, and a capillary temperature of 30 C. Under these conditions, As(V) and orthophosphate ions comigrated. However, the use of a chromate buffer at pH 10, a run voltage of 20 kV, and capillary temperature of 20 C led to complete separation of As(V) and phosphate peaks. Results of these investigations together with recovery test data suggest that separation of the As species from soil solutions can be achieved in less than 5 min with detection limits of 0.50, 0.10, and 0.10 mg L{sup {minus}1} for As(III), As(V), and DMA, respectively.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
CSIRO Land and Water and the Cooperative Research Centre for Soil and Land Management, Glen Osmond, South Australia (AU)
OSTI Identifier:
20075800
Resource Type:
Journal Article
Journal Name:
Soil Science Society of America Journal
Additional Journal Information:
Journal Volume: 64; Journal Issue: 1; Other Information: PBD: Jan-Feb 2000; Journal ID: ISSN 0361-5995
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; SOIL CHEMISTRY; ARSENIC; ELECTROPHORESIS; ANIONS

Citation Formats

Naidu, R, Smith, J, McLaren, R G, Stevens, D P, Sumner, M E, and Jackson, P E. Application of capillary electrophoresis to anion speciation in soil water extracts: 2. Arsenic. United States: N. p., 2000. Web. doi:10.2136/sssaj2000.641122x.
Naidu, R, Smith, J, McLaren, R G, Stevens, D P, Sumner, M E, & Jackson, P E. Application of capillary electrophoresis to anion speciation in soil water extracts: 2. Arsenic. United States. https://doi.org/10.2136/sssaj2000.641122x
Naidu, R, Smith, J, McLaren, R G, Stevens, D P, Sumner, M E, and Jackson, P E. Tue . "Application of capillary electrophoresis to anion speciation in soil water extracts: 2. Arsenic". United States. https://doi.org/10.2136/sssaj2000.641122x.
@article{osti_20075800,
title = {Application of capillary electrophoresis to anion speciation in soil water extracts: 2. Arsenic},
author = {Naidu, R and Smith, J and McLaren, R G and Stevens, D P and Sumner, M E and Jackson, P E},
abstractNote = {A method has been developed for the speciation of arsenic (AsO{sub 2}{sup {minus}}, AsO{sub 4}{sup 3{minus}}, and dimethylarsinic [DMA]) in natural soil solutions from contaminated sites in Australia. The separation of these anions was achieved by capillary zone electrophoresis (CZE) using a fused silica capillary with a basic chromate buffer and on-column indirect UV detection at 254 nm. Method parameters, such as electrolyte pH, run voltage, and capillary temperature were studied in order to establish suitable analytical conditions. The ideal separation for As(III) and DMA was achieved with a buffer pH of 8.0, a run voltage of 25 kV, and a capillary temperature of 30 C. Under these conditions, As(V) and orthophosphate ions comigrated. However, the use of a chromate buffer at pH 10, a run voltage of 20 kV, and capillary temperature of 20 C led to complete separation of As(V) and phosphate peaks. Results of these investigations together with recovery test data suggest that separation of the As species from soil solutions can be achieved in less than 5 min with detection limits of 0.50, 0.10, and 0.10 mg L{sup {minus}1} for As(III), As(V), and DMA, respectively.},
doi = {10.2136/sssaj2000.641122x},
url = {https://www.osti.gov/biblio/20075800}, journal = {Soil Science Society of America Journal},
issn = {0361-5995},
number = 1,
volume = 64,
place = {United States},
year = {2000},
month = {2}
}