Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria

Fife, D J; Bruhn, D F; Miller, K S; Stoner, D L

doi:10.1128/AEM.66.5.2208-2210.2000

Title: Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria

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Abstract

A fluorescence-labeled wheat germ agglutinin staining technique was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.

Authors:: Fife, D J; Bruhn, D F; Miller, K S; Stoner, D L

Publication Date:: Mon May 01 00:00:00 EDT 2000

Research Org.:: Idaho National Lab. (INL), Idaho Falls, ID (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 20075765

DOE Contract Number:: AC07-99ID13727

Resource Type:: Journal Article

Journal Name:: Applied and Environmental Microbiology

Additional Journal Information:: Journal Volume: 66; Journal Issue: 5; Other Information: PBD: May 2000; Journal ID: ISSN 0099-2240

Country of Publication:: United States

Language:: English

Subject:: 01 COAL, LIGNITE, AND PEAT; 54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; IRON; SULFATES; PYRITE; EVALUATION; FLUORESCENCE; STAINS; CYTOLOGICAL TECHNIQUES; BACTERIA; SOILS

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                    Fife, D J, Bruhn, D F, Miller, K S, and Stoner, D L. Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria.  United States: N. p., 2000. 
        Web.  doi:10.1128/AEM.66.5.2208-2210.2000.

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                    Fife, D J, Bruhn, D F, Miller, K S, & Stoner, D L. Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria.  United States.  https://doi.org/10.1128/AEM.66.5.2208-2210.2000

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                    Fife, D J, Bruhn, D F, Miller, K S, and Stoner, D L. 2000.  
        "Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria".  United States.  https://doi.org/10.1128/AEM.66.5.2208-2210.2000.

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@article{osti_20075765,

  title        = {Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria},

  author       = {Fife, D J and Bruhn, D F and Miller, K S and Stoner, D L},

  abstractNote = {A fluorescence-labeled wheat germ agglutinin staining technique was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.},

  doi          = {10.1128/AEM.66.5.2208-2210.2000},

  url          = {https://www.osti.gov/biblio/20075765},
  journal      = {Applied and Environmental Microbiology},

  issn         = {0099-2240},

  number       = 5,

  volume       = 66,

  place        = {United States},

  year         = {Mon May 01 00:00:00 EDT 2000},

  month        = {Mon May 01 00:00:00 EDT 2000}

}

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https://doi.org/10.1128/AEM.66.5.2208-2210.2000

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