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Title: Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria

Abstract

A fluorescence-labeled wheat germ agglutinin staining technique was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.

Authors:
; ; ;
Publication Date:
Research Org.:
Idaho National Engineering and Environmental Lab., Idaho Falls, ID (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
20075765
DOE Contract Number:  
AC07-99ID13727
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 66; Journal Issue: 5; Other Information: PBD: May 2000; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
01 COAL, LIGNITE, AND PEAT; 54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; IRON; SULFATES; PYRITE; EVALUATION; FLUORESCENCE; STAINS; CYTOLOGICAL TECHNIQUES; BACTERIA; SOILS

Citation Formats

Fife, D.J., Bruhn, D.F., Miller, K.S., and Stoner, D.L. Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria. United States: N. p., 2000. Web. doi:10.1128/AEM.66.5.2208-2210.2000.
Fife, D.J., Bruhn, D.F., Miller, K.S., & Stoner, D.L. Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria. United States. doi:10.1128/AEM.66.5.2208-2210.2000.
Fife, D.J., Bruhn, D.F., Miller, K.S., and Stoner, D.L. Mon . "Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria". United States. doi:10.1128/AEM.66.5.2208-2210.2000.
@article{osti_20075765,
title = {Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria},
author = {Fife, D.J. and Bruhn, D.F. and Miller, K.S. and Stoner, D.L.},
abstractNote = {A fluorescence-labeled wheat germ agglutinin staining technique was modified and found to be effective for staining gram-positive, acidophilic mining bacteria. Bacteria identified by others as being gram positive through 16S rRNA sequence analyses, yet clustering near the divergence of that group, stained weakly. Gram-negative bacteria did not stain. Background staining of environmental samples was negligible, and pyrite and soil particles in the samples did not interfere with the staining procedure.},
doi = {10.1128/AEM.66.5.2208-2210.2000},
journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 5,
volume = 66,
place = {United States},
year = {2000},
month = {5}
}