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Title: Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR

Abstract

Unculturable polycyclic aromatic hydrocarbon (PAH)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight PAHs. The population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based studies. The authors used the recently described phn genes of Burkholderia sp. strain RP007, which feature only rarely in culture-based studies, as an alternative genotype for naphthalene and phenanthrene degradation and compared this genotype with the genotypically distinct but ubiquitous nah-like class in different soils. Competitive PCR quantification of phnAc and nahAc, which encode the iron sulfur protein large ({alpha}) subunits of PAH dioxygenases in nah-like and phn catabolic operons, revealed that the phn genotype can have a greater ecological significance than the nah-like genotype.

Authors:
;
Publication Date:
Research Org.:
Landcare Research, Hamilton (NZ)
OSTI Identifier:
20075756
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 66; Journal Issue: 5; Other Information: PBD: May 2000; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; SOILS; LAND POLLUTION; NEW ZEALAND; NAPHTHALENE; PHENANTHRENE; GENES; GENOTYPE; POLYMERASE CHAIN REACTION; BACTERIA; BIODEGRADATION; REMEDIAL ACTION

Citation Formats

Laurie, A.D., and Lloyd-Jones, G. Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR. United States: N. p., 2000. Web. doi:10.1128/AEM.66.5.1814-1817.2000.
Laurie, A.D., & Lloyd-Jones, G. Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR. United States. doi:10.1128/AEM.66.5.1814-1817.2000.
Laurie, A.D., and Lloyd-Jones, G. Mon . "Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR". United States. doi:10.1128/AEM.66.5.1814-1817.2000.
@article{osti_20075756,
title = {Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR},
author = {Laurie, A.D. and Lloyd-Jones, G.},
abstractNote = {Unculturable polycyclic aromatic hydrocarbon (PAH)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight PAHs. The population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based studies. The authors used the recently described phn genes of Burkholderia sp. strain RP007, which feature only rarely in culture-based studies, as an alternative genotype for naphthalene and phenanthrene degradation and compared this genotype with the genotypically distinct but ubiquitous nah-like class in different soils. Competitive PCR quantification of phnAc and nahAc, which encode the iron sulfur protein large ({alpha}) subunits of PAH dioxygenases in nah-like and phn catabolic operons, revealed that the phn genotype can have a greater ecological significance than the nah-like genotype.},
doi = {10.1128/AEM.66.5.1814-1817.2000},
journal = {Applied and Environmental Microbiology},
issn = {0099-2240},
number = 5,
volume = 66,
place = {United States},
year = {2000},
month = {5}
}