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Title: Metabolism of fluoranthene in different plant cell cultures and intact plants

Abstract

The metabolism of fluoranthene was investigated in 11 cell cultures of different plant species using a [{sup 14}C]-labeled standard. Most species metabolized less than 5% of fluoranthene to soluble metabolites and formed less than 5% nonextractable residues during the standardized 48-h test procedure. Higher metabolic rates were observed in lettuce (Lactuca sativa, 6%), wheat (Tricitum aestivum, 9%), and tomato (Lycopersicon esculentum, 15%). A special high metabolic rate of nearly 50% was determined for the rose species Paul's Scarlet. Chromatographic analysis of metabolites extracted from aseptically grown tomato plants proved that the metabolites detected in the cell cultures were also formed in the intact plants. Metabolites produced in tomato and rose cells from [{sup 14}C]-fluoranthene were conjugated with glucose, glucuronic acid, and other cell components. After acid hydrolyses, the main metabolite of both species was 1-hydroxyfluoranthene as identified by gas chromatography-mass spectrometry and high-performance liquid chromatography with diode array detection. The second metabolite formed by both species was 8-hydroxyfluoranthene. A third metabolite in tomatoes was 3-hydroxyfluoranthene.

Authors:
;
Publication Date:
Research Org.:
Bundesforschungsanstalt fuer Landwirtschaft, Braunschweig (DE)
OSTI Identifier:
20067669
Alternate Identifier(s):
OSTI ID: 20067669
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 19; Journal Issue: 5; Other Information: PBD: May 2000; Journal ID: ISSN 0730-7268
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; SOILS; BIODEGRADATION; CONDENSED AROMATICS; METABOLISM; GENETIC VARIABILITY; PLANTS; CELL CULTURES

Citation Formats

Kolb, M., and Harms, H. Metabolism of fluoranthene in different plant cell cultures and intact plants. United States: N. p., 2000. Web. doi:10.1897/1551-5028(2000)019<1304:MOFIDP>2.3.CO;2.
Kolb, M., & Harms, H. Metabolism of fluoranthene in different plant cell cultures and intact plants. United States. doi:10.1897/1551-5028(2000)019<1304:MOFIDP>2.3.CO;2.
Kolb, M., and Harms, H. Mon . "Metabolism of fluoranthene in different plant cell cultures and intact plants". United States. doi:10.1897/1551-5028(2000)019<1304:MOFIDP>2.3.CO;2.
@article{osti_20067669,
title = {Metabolism of fluoranthene in different plant cell cultures and intact plants},
author = {Kolb, M. and Harms, H.},
abstractNote = {The metabolism of fluoranthene was investigated in 11 cell cultures of different plant species using a [{sup 14}C]-labeled standard. Most species metabolized less than 5% of fluoranthene to soluble metabolites and formed less than 5% nonextractable residues during the standardized 48-h test procedure. Higher metabolic rates were observed in lettuce (Lactuca sativa, 6%), wheat (Tricitum aestivum, 9%), and tomato (Lycopersicon esculentum, 15%). A special high metabolic rate of nearly 50% was determined for the rose species Paul's Scarlet. Chromatographic analysis of metabolites extracted from aseptically grown tomato plants proved that the metabolites detected in the cell cultures were also formed in the intact plants. Metabolites produced in tomato and rose cells from [{sup 14}C]-fluoranthene were conjugated with glucose, glucuronic acid, and other cell components. After acid hydrolyses, the main metabolite of both species was 1-hydroxyfluoranthene as identified by gas chromatography-mass spectrometry and high-performance liquid chromatography with diode array detection. The second metabolite formed by both species was 8-hydroxyfluoranthene. A third metabolite in tomatoes was 3-hydroxyfluoranthene.},
doi = {10.1897/1551-5028(2000)019<1304:MOFIDP>2.3.CO;2},
journal = {Environmental Toxicology and Chemistry},
issn = {0730-7268},
number = 5,
volume = 19,
place = {United States},
year = {2000},
month = {5}
}