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Title: Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods

Abstract

Acute toxicity and genotoxicity of cadmium, copper, chromium, mercury, nickel, and zinc dissolved in deionized water and in sewage were established by comparing the EC50 and EC20 values obtained by different microbial assays. For acute toxicity determination. The Netherlands Standard NEN6509 test, the spectrophotometric assays of respiratory inhibition using Saccharmyces cerevisiae and Pseudomonas fluorescens, and the Microtox' test were employed. To determine metal genotoxicity, the Salmonella typhimurium and Escherichia coli mutagenicity tests, the SOS-{beta}-galactosidase genotoxicity test, and the Mutatox{trademark} assay were used. The toxicity of the different assayed metals varied from the most toxic, mercury, to the least toxic, nickel and zinc. Two different rankings of toxicity and genotoxicity, very similar to each other, were established. The toxicity ranking was Hg > Cr > Cd {approximately} Cu {approximately} n > Ni, and the genotoxicity ranking was Hg > Cr > Cu {approximately} Cd {approximately} Ni > Zn. The association between the toxicity and genotoxicity of copper and chromium in the dissolved and suspended fractions of sewage was also determined. Copper was mainly associated with the suspended fractions and chromium with the dissolved fractions of sewage.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. de Malaga (ES)
OSTI Identifier:
20062540
Alternate Identifier(s):
OSTI ID: 20062540
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 19; Journal Issue: 6; Other Information: PBD: Jun 2000; Journal ID: ISSN 0730-7268
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; WATER POLLUTION; CADMIUM; COPPER; CHROMIUM; MERCURY; NICKEL; ZINC; SEWAGE; BIOASSAY; BACTERIA

Citation Formats

Codina, J.C., Cazorla, F.M., Perez-Garcia, A., and De Vicente, A. Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods. United States: N. p., 2000. Web. doi:10.1897/1551-5028(2000)019<1552:HMTAGI>2.3.CO;2.
Codina, J.C., Cazorla, F.M., Perez-Garcia, A., & De Vicente, A. Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods. United States. doi:10.1897/1551-5028(2000)019<1552:HMTAGI>2.3.CO;2.
Codina, J.C., Cazorla, F.M., Perez-Garcia, A., and De Vicente, A. Thu . "Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods". United States. doi:10.1897/1551-5028(2000)019<1552:HMTAGI>2.3.CO;2.
@article{osti_20062540,
title = {Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods},
author = {Codina, J.C. and Cazorla, F.M. and Perez-Garcia, A. and De Vicente, A.},
abstractNote = {Acute toxicity and genotoxicity of cadmium, copper, chromium, mercury, nickel, and zinc dissolved in deionized water and in sewage were established by comparing the EC50 and EC20 values obtained by different microbial assays. For acute toxicity determination. The Netherlands Standard NEN6509 test, the spectrophotometric assays of respiratory inhibition using Saccharmyces cerevisiae and Pseudomonas fluorescens, and the Microtox' test were employed. To determine metal genotoxicity, the Salmonella typhimurium and Escherichia coli mutagenicity tests, the SOS-{beta}-galactosidase genotoxicity test, and the Mutatox{trademark} assay were used. The toxicity of the different assayed metals varied from the most toxic, mercury, to the least toxic, nickel and zinc. Two different rankings of toxicity and genotoxicity, very similar to each other, were established. The toxicity ranking was Hg > Cr > Cd {approximately} Cu {approximately} n > Ni, and the genotoxicity ranking was Hg > Cr > Cu {approximately} Cd {approximately} Ni > Zn. The association between the toxicity and genotoxicity of copper and chromium in the dissolved and suspended fractions of sewage was also determined. Copper was mainly associated with the suspended fractions and chromium with the dissolved fractions of sewage.},
doi = {10.1897/1551-5028(2000)019<1552:HMTAGI>2.3.CO;2},
journal = {Environmental Toxicology and Chemistry},
issn = {0730-7268},
number = 6,
volume = 19,
place = {United States},
year = {2000},
month = {6}
}