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Title: Method for facilitating the introduction of material into cells

Abstract

The present invention is a method for creating a localized disruption within a boundary of a cell or structure by exposing a boundary of a cell or structure to a set of energetically charged particles. The energy of the charged particles is regulated so that the charged particles have an amount of kinetic energy sufficient to create a localized disruption within an area of the boundary of the cell or structure. Upon creation of the localized disruption, the amount of kinetic energy decreases to an amount insufficient to create further damage within the cell or structure beyond the boundary. The present invention is also a method for facilitating the introduction of a material into a cell or structure using the same methodology. The area of the boundary of the cell or structure is further excited where the localized disruption was created causing a localized temporary opening within the boundary. Material is then introduced through the temporary opening into the cell or structure.

Inventors:
;
Publication Date:
Research Org.:
US Department of Energy, Washington, DC (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
20050619
Application Number:
Int. Cl. C12N 15/00; C12N 15/85; C12Q 1/68; US 6,060,315/A/; 9-152,926; TRN: IM200024%%130
Assignee:
PTO; EDB-00:033225
DOE Contract Number:  
AC05-96OR22464
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 9 May 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CYTOLOGY; PLANT CELLS; CYTOLOGICAL TECHNIQUES; KINETIC ENERGY

Citation Formats

Holcomb, D.E., and McKnight, T.E. Method for facilitating the introduction of material into cells. United States: N. p., 2000. Web.
Holcomb, D.E., & McKnight, T.E. Method for facilitating the introduction of material into cells. United States.
Holcomb, D.E., and McKnight, T.E. Tue . "Method for facilitating the introduction of material into cells". United States.
@article{osti_20050619,
title = {Method for facilitating the introduction of material into cells},
author = {Holcomb, D.E. and McKnight, T.E.},
abstractNote = {The present invention is a method for creating a localized disruption within a boundary of a cell or structure by exposing a boundary of a cell or structure to a set of energetically charged particles. The energy of the charged particles is regulated so that the charged particles have an amount of kinetic energy sufficient to create a localized disruption within an area of the boundary of the cell or structure. Upon creation of the localized disruption, the amount of kinetic energy decreases to an amount insufficient to create further damage within the cell or structure beyond the boundary. The present invention is also a method for facilitating the introduction of a material into a cell or structure using the same methodology. The area of the boundary of the cell or structure is further excited where the localized disruption was created causing a localized temporary opening within the boundary. Material is then introduced through the temporary opening into the cell or structure.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {5}
}