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Title: Method and apparatus for staining immobilized nucleic acids

Abstract

A method for staining immobilized nucleic acids includes the following steps: affixing DNA probes to a solid substrate; moving target DNA material into proximity with the DNA probes, whereby the target DNA hybridized with specific ones of the DNA probes; and moving a fluorescent dye into proximity with the hybridized target DNA, whereby the fluorescent dye binds to the hybridized DNA to enable subsequent detection of fluorescence.

Inventors:
; ;
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN
Sponsoring Org.:
USDOE
OSTI Identifier:
20050616
Application Number:
Int. Cl. G01N 27/26; US 6,056,859/A/; 8-800,241; TRN: IM200024%%127
Assignee:
ORNL; EDB-00:033222
DOE Contract Number:  
AC05-84OR21400
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 2 May 2000
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA; DNA HYBRIDIZATION; FLUORESCENCE; STAINS; DYES

Citation Formats

Ramsey, J.M., Foote, R.S., and Jacobson, S.C. Method and apparatus for staining immobilized nucleic acids. United States: N. p., 2000. Web.
Ramsey, J.M., Foote, R.S., & Jacobson, S.C. Method and apparatus for staining immobilized nucleic acids. United States.
Ramsey, J.M., Foote, R.S., and Jacobson, S.C. Tue . "Method and apparatus for staining immobilized nucleic acids". United States.
@article{osti_20050616,
title = {Method and apparatus for staining immobilized nucleic acids},
author = {Ramsey, J.M. and Foote, R.S. and Jacobson, S.C.},
abstractNote = {A method for staining immobilized nucleic acids includes the following steps: affixing DNA probes to a solid substrate; moving target DNA material into proximity with the DNA probes, whereby the target DNA hybridized with specific ones of the DNA probes; and moving a fluorescent dye into proximity with the hybridized target DNA, whereby the fluorescent dye binds to the hybridized DNA to enable subsequent detection of fluorescence.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {5}
}