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Title: Ethanol production from xylose with the yeast Pichia stipitis and simultaneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm (GLSA)

Abstract

The bioconversion of xylose into ethanol with the yeast Pichia stipitis CBS 5773 is inhibited when 20 g/L of ethanol are present in the fermentation broth. In order to avoid this limitation, the fermentation was carried out with simultaneous recovery of product by CO{sub 2} stripping. The fermentation was also improved by attaching a side-arm to the main body of a classical gas-lift loop fermentor. This side-arm increases the liquid circulation, mass transfer, and gas distribution, reducing the amount of oxygen in the inlet gas necessary to perform the fermentation of xylose under microaerobic conditions (K{sub L}a{approx_equal} 16 h{sup {minus}1}). The continuous stripping of ethanol from the fermentation broth in this new bioreactor system allowed the consumption of higher xylose concentrations than using Erlenmeyer shaker flasks, improved significantly the process productivity and provided a clean ethanol solution by using an ice-cooled condenser system. Finally, a fed-batch fermentation was carried out with a K{sub L}a = 15.8 h{sup {minus}1}. Starting with 248.2 g of xylose, 237.6 g of xylose was consumed to produce 88.1 g of ethanol which represents 72.6% of the theoretical yield.

Authors:
; ; ;
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (US)
OSTI Identifier:
20018974
Resource Type:
Journal Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 67; Journal Issue: 3; Other Information: PBD: 5 Feb 2000; Journal ID: ISSN 0006-3592
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ETHANOL; XYLOSE; BIOSYNTHESIS; FERMENTATION; YEASTS; YIELDS; BIOREACTORS; MATERIALS RECOVERY

Citation Formats

Dominguez, J M, Cao, N, Gong, C S, and Tsao, G T. Ethanol production from xylose with the yeast Pichia stipitis and simultaneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm (GLSA). United States: N. p., 2000. Web. doi:10.1002/(SICI)1097-0290(20000205)67:3<336::AID-BIT10>3.0.CO;2-6.
Dominguez, J M, Cao, N, Gong, C S, & Tsao, G T. Ethanol production from xylose with the yeast Pichia stipitis and simultaneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm (GLSA). United States. doi:10.1002/(SICI)1097-0290(20000205)67:3<336::AID-BIT10>3.0.CO;2-6.
Dominguez, J M, Cao, N, Gong, C S, and Tsao, G T. Sat . "Ethanol production from xylose with the yeast Pichia stipitis and simultaneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm (GLSA)". United States. doi:10.1002/(SICI)1097-0290(20000205)67:3<336::AID-BIT10>3.0.CO;2-6.
@article{osti_20018974,
title = {Ethanol production from xylose with the yeast Pichia stipitis and simultaneous product recovery by gas stripping using a gas-lift loop fermentor with attached side-arm (GLSA)},
author = {Dominguez, J M and Cao, N and Gong, C S and Tsao, G T},
abstractNote = {The bioconversion of xylose into ethanol with the yeast Pichia stipitis CBS 5773 is inhibited when 20 g/L of ethanol are present in the fermentation broth. In order to avoid this limitation, the fermentation was carried out with simultaneous recovery of product by CO{sub 2} stripping. The fermentation was also improved by attaching a side-arm to the main body of a classical gas-lift loop fermentor. This side-arm increases the liquid circulation, mass transfer, and gas distribution, reducing the amount of oxygen in the inlet gas necessary to perform the fermentation of xylose under microaerobic conditions (K{sub L}a{approx_equal} 16 h{sup {minus}1}). The continuous stripping of ethanol from the fermentation broth in this new bioreactor system allowed the consumption of higher xylose concentrations than using Erlenmeyer shaker flasks, improved significantly the process productivity and provided a clean ethanol solution by using an ice-cooled condenser system. Finally, a fed-batch fermentation was carried out with a K{sub L}a = 15.8 h{sup {minus}1}. Starting with 248.2 g of xylose, 237.6 g of xylose was consumed to produce 88.1 g of ethanol which represents 72.6% of the theoretical yield.},
doi = {10.1002/(SICI)1097-0290(20000205)67:3<336::AID-BIT10>3.0.CO;2-6},
journal = {Biotechnology and Bioengineering},
issn = {0006-3592},
number = 3,
volume = 67,
place = {United States},
year = {2000},
month = {2}
}