skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens

Abstract

Hairy roots were used to investigate cadmium uptake by Thlaspi caerulescens, a metal hyperaccumulator plant with potential applications in phytoremediation and phytomining. Experiments were carried out in nutrient media under conditions supporting root growth. Accumulation of Cd in short-term (9-h) experiments varied with initial medium pH and increased after treating the roots with H{sup +}-ATPase inhibitor. The highest equilibrium Cd content measured in T. caerulescens roots was 62,800 {micro}g g{sup {minus}1} dry weight, or 6.3% dry weight, at a liquid Cd concentration of 3,710 ppm. Cd levels in live T. caerulescens roots were 1.5- to 1.7-fold those in hairy roots of nonhyperaccumulator species exposed to the same Cd concentration, but similar to the Cd content of auto-claved T. caerulescens roots. The ability to grow at Cd concentrations of up to 100 ppm clearly distinguished T. caerulescens hairy roots from the nonhyperaccumulators. The specific growth rate of T. caerulescens roots was essentially unaffected by 20 to 50 ppm Cd in the culture medium; in contrast, N. tabacum roots turned dark brown at 20 ppm and growth was negligible. Up to 10,600 {micro}g g{sup {minus}1} dry weight Cd was accumulated by growing T. caerulescens hairy roots. Measurement of Cd levels in whilemore » roots and in the cell wall fraction revealed significant differences in the responses of T. caerulescens and N. tabacum roots to 20 ppm Cd. Most metal was transported directly into the symplasm of N. tabacum roots within 3 days of exposure; in contrast, T. caerulescens roots stored virtually all of their Cd in the wall fraction for the first 7 to 10 days. This delay in transmembrane uptake may represent an important defensive strategy against Cd poisoning in T. caerulescens, allowing time for activation of intracellular mechanisms for heavy metal detoxification.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of New South Wales, Sydney (AU)
OSTI Identifier:
20018972
Resource Type:
Journal Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 67; Journal Issue: 5; Other Information: PBD: 5 Mar 2000; Journal ID: ISSN 0006-3592
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; REMEDIAL ACTION; CADMIUM; BIOLOGICAL ACCUMULATION; PLANTS; ROOT ABSORPTION; LAND POLLUTION CONTROL

Citation Formats

Nedelkoska, T V, and Doran, P M. Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens. United States: N. p., 2000. Web. doi:10.1002/(SICI)1097-0290(20000305)67:5<607::AID-BIT11>3.3.CO;2-V.
Nedelkoska, T V, & Doran, P M. Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens. United States. https://doi.org/10.1002/(SICI)1097-0290(20000305)67:5<607::AID-BIT11>3.3.CO;2-V
Nedelkoska, T V, and Doran, P M. 2000. "Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens". United States. https://doi.org/10.1002/(SICI)1097-0290(20000305)67:5<607::AID-BIT11>3.3.CO;2-V.
@article{osti_20018972,
title = {Hyperaccumulation of cadmium by hairy roots of Thlaspi caerulescens},
author = {Nedelkoska, T V and Doran, P M},
abstractNote = {Hairy roots were used to investigate cadmium uptake by Thlaspi caerulescens, a metal hyperaccumulator plant with potential applications in phytoremediation and phytomining. Experiments were carried out in nutrient media under conditions supporting root growth. Accumulation of Cd in short-term (9-h) experiments varied with initial medium pH and increased after treating the roots with H{sup +}-ATPase inhibitor. The highest equilibrium Cd content measured in T. caerulescens roots was 62,800 {micro}g g{sup {minus}1} dry weight, or 6.3% dry weight, at a liquid Cd concentration of 3,710 ppm. Cd levels in live T. caerulescens roots were 1.5- to 1.7-fold those in hairy roots of nonhyperaccumulator species exposed to the same Cd concentration, but similar to the Cd content of auto-claved T. caerulescens roots. The ability to grow at Cd concentrations of up to 100 ppm clearly distinguished T. caerulescens hairy roots from the nonhyperaccumulators. The specific growth rate of T. caerulescens roots was essentially unaffected by 20 to 50 ppm Cd in the culture medium; in contrast, N. tabacum roots turned dark brown at 20 ppm and growth was negligible. Up to 10,600 {micro}g g{sup {minus}1} dry weight Cd was accumulated by growing T. caerulescens hairy roots. Measurement of Cd levels in while roots and in the cell wall fraction revealed significant differences in the responses of T. caerulescens and N. tabacum roots to 20 ppm Cd. Most metal was transported directly into the symplasm of N. tabacum roots within 3 days of exposure; in contrast, T. caerulescens roots stored virtually all of their Cd in the wall fraction for the first 7 to 10 days. This delay in transmembrane uptake may represent an important defensive strategy against Cd poisoning in T. caerulescens, allowing time for activation of intracellular mechanisms for heavy metal detoxification.},
doi = {10.1002/(SICI)1097-0290(20000305)67:5<607::AID-BIT11>3.3.CO;2-V},
url = {https://www.osti.gov/biblio/20018972}, journal = {Biotechnology and Bioengineering},
issn = {0006-3592},
number = 5,
volume = 67,
place = {United States},
year = {Sun Mar 05 00:00:00 EST 2000},
month = {Sun Mar 05 00:00:00 EST 2000}
}