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Title: Streptavidin mutants

Abstract

The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.

Publication Date:
Research Org.:
US Department of Energy, Washington, DC (US)
Sponsoring Org.:
USDOE
OSTI Identifier:
20013269
Application Number:
Int. Cl. C07K 14/36; US 6,022,951/A/; 8-628,540; TRN: IM200010%%137
Assignee:
PTO; EDB-00:006690
DOE Contract Number:  
FG02-93ER61656
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 8 Feb 2000
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PROTEINS; AFFINITY; BIOCHEMICAL REACTION KINETICS; BIOTIN; NUCLEIC ACIDS

Citation Formats

. Streptavidin mutants. United States: N. p., 2000. Web.
. Streptavidin mutants. United States.
. Tue . "Streptavidin mutants". United States.
@article{osti_20013269,
title = {Streptavidin mutants},
author = {},
abstractNote = {The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2000},
month = {2}
}