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Title: Transcriptional enhancer from milk protein genes

Abstract

The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.

Inventors:
; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA
Sponsoring Org.:
USDOE
OSTI Identifier:
20013258
Application Number:
Int. Cl. C12N 5/10; C12N 1/00; C12N 15/11; C12N 15/79; US 6,004,805/A/; 7-891,541; TRN: IM200010%%126
Assignee:
LBNL; EDB-00:006679
DOE Contract Number:
AC03-76SF00098
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 21 Dec 1999
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; TRANSCRIPTION; DNA; CELL CULTURES; MILK; PROTEINS; GENES; GENE REGULATION; NUCLEOTIDES

Citation Formats

Casperson, G.F., Schmidhauser, C.T., and Bissell, M.J.. Transcriptional enhancer from milk protein genes. United States: N. p., 1999. Web.
Casperson, G.F., Schmidhauser, C.T., & Bissell, M.J.. Transcriptional enhancer from milk protein genes. United States.
Casperson, G.F., Schmidhauser, C.T., and Bissell, M.J.. 1999. "Transcriptional enhancer from milk protein genes". United States. doi:.
@article{osti_20013258,
title = {Transcriptional enhancer from milk protein genes},
author = {Casperson, G.F. and Schmidhauser, C.T. and Bissell, M.J.},
abstractNote = {The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1999,
month =
}
  • The invention relates to novel enhancer nucleotide sequences which stimulate transcription of heterologous DNA in cells in culture. The enhancers are derived from major milk protein genes by the process of deletion mapping and functional analysis. The invention also relates to expression vectors containing the novel enhancers.
  • The invariant chain (I{sub i}) is a glycoprotein coexpressed with the major histocompatibility complex (MHC) class II antigens. Although I{sub i} is encoded by a single gene unlinked to the MHC gene complex, I{sub i} and MHC class II appear to have similar patterns of tissue specific expression and generally are coordinately regulated by cytokines. The authors present evidence that transcription of the murine I{sub i} gene is controlled by multiple cis-acting elements. The 5{prime} regulatory region of the I{sub i} gene appears to be combined of conserved class II regulatory elements with promoter elements (H Box, X box, andmore » modified Y box) serves as an upstream enhancer in the I{sub i} gene and might contribute to the coexpression of MHC class II and I{sub i} genes.« less
  • Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
  • Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.
  • Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of modulating transcription or transcription or transcriptional control using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.